Government shoots down AR-15 petition

first_imgThe local MP also believes the outcome of the US Presidential Election this fall could have an impact on the issue going forward. FORT ST. JOHN, B.C. – It carried more than 25,000 signatures, but the Trudeau government has shot down the AR-15 e-petition launched by Marc Bennett of Corner Brook, Newfoundland, and sponsored by Prince George/Peace River/Northern Rockies MP, Bob Zimmer.This is the petition tabled in the Commons this summer calling on the government to reclassify the rifle as an unrestricted firearm.The petitioners wrote, “This rifle is the most versatile hunting rifle in the world”…adding…“The calibre can easily be changed to meet lawful provincial hunting requirements from small game to big game animals, without the need to purchase multiple gaming rifles .”- Advertisement -However, the Liberal government was having no part of their arguments with Public Safety Minister Ralph Goodale saying he has no intention of invoking a section of Canada’s criminal code to reclassify the firearm as unrestricted.While not surprised Mr. Zimmer did find the government response troubling, especially Mr. Goodale’s commitment to put the decision making authority about weapons classification back into the hands of RCMP.However, Canada’s restricted gun ownership increased nine and half percent last year and Mr. Zimmer says the firearms debate is far from over.Advertisementlast_img read more

Ison and Kennedy Lead Bulldog on Final Day at Loyola Intercollegiate

first_img GOODYEAR, Ariz. – The Drake University men’s finished in 15th-place at the Loyola Intercollegiate on Monday in Goodyear, Ariz. The Bulldogs concluded their season opening tournament with a total score of 884 (293-297-294). Connecticut took home the team title with a score of 834 (282-278-274). Freshman Chase Wicklund, who competed as an individual, registered a total score of 220 (72-73-75). The Bulldogs return to action on March 6 when they play in the Fort Lauderdale Intercollegiate at Ft. Lauderdale, Fla. Print Friendly Version “It was nice to see some putts drop as we made 25 birdies which was more than the first two days combine. We had a few big numbers that really hurt us today,” said Drake head coach Matt Lewis.Individually, Drew Ison and Jack Kennedy each fired a one-over par, 73 on the final day. Ison placed tied for 30th with a total score of 215 (71-71-73), while Kennedy totaled a 223 (75-75-73). Matt Lavery also registered a 54-hole total of 223 (74-75-74) after shooting a two-over 74 in the final round. Tommi Avant posted a total score of 226 (73-76-77), while Arjun Reddy recorded his best round of the tournament, shooting a 74 for a total score of 232 (79-79-74).center_img “For the first tournament in four months we definitely learned a lot about where our games are. This will be helpful going into next week’s tournament,” said Lewis. Story Links Results last_img read more

Sponsorship boost for SA tennis

first_img11 June 2009The South African Tennis Association has received a welcome boost, with the signing of a three-year, R8-million sponsorship deal with medical scheme Keyhealth.The money will be geared towards Davis Cup, junior tennis, and development. Earlier this year, KeyHealth partnered with the South African Tennis Association (Sata) in the SA Open and Davis Cup ties.“This is an absolute win for tennis in South Africa,” Sata chief executive Ian Smith said in a statement this week. “To have KeyHealth as a partner in the resurrection of the sport locally is what SA tennis has been lacking for some time.”‘A growing sport’KeyHealth marketing chairman Peter Sharman explained: “Tennis is a growing sport in South Africa, and we were fortunate to make contact with SA Tennis some 12 months ago and provide them with a small sponsorship to assist them in their efforts to promote tennis throughout the country.“The board of KeyHealth then took the decision to become more involved, especially as there was a need to promote tennis to the underprivileged and provide them with a healthy sporting opportunity on an individual basis,” Sharman said.“Through this initiative we hope to create primary healthcare awareness and a healthy lifestyle for all who are involved, and through the publicity it generates we will bring that awareness to the masses.”Improvement and expansionIn recent years South African tennis has shown encouraging signs of improvement and expansion.The South African Open was revived in 2007 after the country had last hosted an ATP event in 1994. The tournament that year was a challenger event, but in 2009 it enjoyed full ATP Tour status and drew a quality field.Also this year, two challenger tournaments, for men and women, were held at the Arthur Ashe Tennis Complex in Soweto, Johannesburg. It was the first time that world class tennis had been taken to the world famous township.In September, South Africa will face India at home for a place in the World Group of the Davis Cup, the highest level of the international competition. This comes on the back of 10 successive wins in the event since 2006.At the junior level, Chanel Simmonds recently made it to the quarterfinals of the girls’ French Open.Clearly, the trend is up for South African tennis, and a healthy sponsorship will no doubt help stimulate the sport and expand it throughout the country.SAinfo reporter and South African Tennis AssociationWould you like to use this article in your publication or on your website? See: Using SAinfo materiallast_img read more

Kanyamazane Government Senior Citizens – Episode 22 – Play Your Part

first_imgThe next episode of the Play Your Part Television Series will air on Saturday, 17 February 2018 on SABC 2 at 18h00.Episode 22 is aligned to health, innovation, and active citizenship in respect of volunteerism.It features the Kanyamazane Government Senior Citizens Social Club, Human Writes social innovation start-up and Ikamva Labantu charity organisaton, the Hands of Compassion Christian Community and University of Free State student, Andile Putu’s involvement in the Enactus Entrepreneurial Community.The Kanyamazane Government Senior Citizens Social Club, is a gathering of retired public servants who use their knowledge on health and wellness to assist in the health care of citizens in the community.The Human writ.es social innovation start-up improves child and adult literacy through the Ikamva Labantu charity organisation based in Cape Town.Under the guidance of Pastors Alan and Xana MacCauley, the Johannesburg based Hands of Compassion Christian Community affords second chances to young people.Andile Putu oversaw the Enactus Entrepreneurial Community while he was a student at the University of the Free State in an attempt to assist HIV positive orphans and reviving the manufacturing industry in the Free State province.Play Your Part is broadcast at 18:00 on every Saturday on SABC2.To get involved in playing your part in South Africa:Check out the conversation on Twitter: #GetInvolvedTell us how you Play Your Part through our social media channels:Follow us on Twitter: @PlayYourPartSA;Follow Brand South Africa on Twitter: @Brand_SA; orLike us on Facebook: Official Brand South Africalast_img read more

AWCC: Rebisco PSL-Manila yields to Hisamitsu Springs

first_imgLakers win 9th straight, hold off Pelicans ‘Coming Home For Christmas’ is the holiday movie you’ve been waiting for, here’s why South Korea to suspend 25% of coal plants to fight pollution The Japanese, playing together as one team as opposed to the Philippine Superliga selection, really stepped on it in the second and third frames, smartly sensing all their opponents’ weakness and pouncing on just about every unguarded spot on the floor.“We have to have that kind of mindset, which is to play with that kind of speed,” said Vicente.All in all, according to Vicente, the Filipinos played very well and should have learned a neat lesson ahead of their goal to land a medal in the Southeast Asian Games in August.RELATED VIDEOS UST-KAMENOGORSK, Kazakhstan — Hisamitsu Springs needed some time to size up Rebisco PSL-Manila but still came up with a performance worthy of a two-time champion team to post a 25-17, 25-10, 25-14 victory Thursday at the start of the Asian Women’s Club Championships.FEATURED STORIESSPORTSSEA Games: Biñan football stadium stands out in preparedness, completionSPORTSMalditas save PH from shutoutSPORTSPrivate companies step in to help SEA Games hostingThe Filipino spikers, led by 6-foot-5 Jaja Santiago, briefly took control in the first set, 9-6, and kept the game close through her unstoppable attacks and occasional net drops by Kim Fajardo.But that was not enough to keep Rebisco abreast with the 2002 and 2014 titlist which soon employed its almost flawless, and systematic defense at the Boris Alxeandrov Sports Palace. More than 5,000 measles deaths in DR Congo this year — WHO Panelo suggests discounted SEA Games tickets for students PLAY LIST 01:35Panelo suggests discounted SEA Games tickets for students02:49Robredo: True leaders perform well despite having ‘uninspiring’ boss02:42PH underwater hockey team aims to make waves in SEA Games01:44Philippines marks anniversary of massacre with calls for justice01:19Fire erupts in Barangay Tatalon in Quezon City01:07Trump talks impeachment while meeting NCAA athletes Santiago scored 16 points for the Filipinos who are coming into the tournaments without an import. Fajardo had four points and 11 excellent sets, while Ces Molina, Rhea Dimaculangan and Jovelyn Gonzaga each had three points.Mika Reyes and Rachel Anne Daquis both scored two points, while Aiza Pontillas added one for Rebisco which had 18 errors, mostly on attacks.Coach Francis Vicente to Rebisco PSL You didn’t play badly. You just lacked speed and volleyball IQ. Napainit nyo ulo nung Hapon. Be proud. PHOTO BY MARC REYES“Why are you so sad?” asked head coach Francis Vicente during the post-game huddle inside the locker room. “You learned from them. You didn’t play badly. We just need more speed. More volleyball IQ. You got the Japanese coach worried in the first set. Be proud.”Middle blocker Fumika Moriya led the Japan club with 14 points, while Yuka Taura and Yuka Imamura chipped in 12 and 11 markers, respectively.Erika Sakae tossed 33 excellent sets to set Hisamitsu’s offence flowing and wreaking havoc against Rebisco.ADVERTISEMENT Pagasa: Kammuri now a typhoon, may enter PAR by weekend Don’t miss out on the latest news and information. SEA Games: PH beats Indonesia, enters gold medal round in polo Man United fans celebrate triumph after tragedy MOST READ Libero Denden Lazaro assesses the match pic.twitter.com/OXyPFXuHHW— Marc Anthony Reyes (@marcreyesINQ) May 25, 2017ADVERTISEMENT LATEST STORIES Cayetano dares Lacson, Drilon to take lie-detector test: Wala akong kinita sa SEA Games LOOK: Vhong Navarro’s romantic posts spark speculations he’s marrying longtime GF Sports Related Videospowered by AdSparcRead Next View commentslast_img read more

England will not win the World Cup in Brazil: Jamie Carragher

first_imgCarragher also believes that the penalty shoot-outs will also haunt England in the World CupFrom Italia ’90 to Euro 2012, penalty shoot-outs have been England’s undoing in major competitions. Players letting their faithful fans down and ending up in tears on field has been a depressingly common sight. Jamie Carragher, two time World Cupper for England was one of the three players to have his penalty saved by Portugal’s Ricardo Pereira in World Cup 2006 as they were shown the door in the quarter finals.He sat down talking exclusively with India Today to admit the team will continue to be short on confidence in penalties until they win a shoot out in the games that matter. He was also willing to say on record that England don’t have the team to go all the way.Q. Realistically how far do you think England can go this World Cup. They start in a tough group?A. It is a tough group. I still think England will qualify to the next round. But I don’t think they will win the World Cup. They will go to the quarters like they normally do, did so even when I played. If they make it there, they would have still done well.Q. Is it a good or bad thing to run into Italy in the opening game?A. One can look at it both ways. Its a tough start against Italy but England is a tough game for Italy as well. You are straight into the World Cup if you play against one of the top nations. If you do well in a first game that’s tough, it sets you up for the rest of the tournament.advertisementQ. Wayne Rooney. The world looks to him to make it happen. He’s yet to score a goal in World Cup situation. He should have a point to prove?A. Rooney does have a point to prove. He’s scored at all levels, club level, Champions league level. He’s got a good scoring record internationally as well but not at the World Cup and he would be desperate to do well.Q. Inevitably questions are asked about England’s dismal penalty record. You have been through a loss on penalties as well. England would much rather avoid a result on penalty?A. Not just England every team would rather win the game than end in a penalty shoot out but if they are in that situation again, they should try and overcome it. They have lot of good penalty takers in Gerrard, Lambert, Baines, Lampard; but until they win one they will be short of confidence.Q. Who are the favorites to lift the trophy in your book? And for the Golden boot?A. Brazil are the favourites being the home team. Maybe the home advantage and crowd will roar them on to victory. Spain is the other team you can fancy. For the golden boot, hopefully it will be Messi. I hope he has a great World Cup because he deserves itQ. No European team has won a World Cup in South America. Do you see it changing this time?A. Spain and Germany are the two European teams to break the deadlock of Europeans never having won in South America. But Brazil and Argentina have a greater chance. I still fancy Brazil to win.last_img read more

Half of First Nations children living in poverty should shock Canadians report

first_imgAPTN National NewsHalf of First Nations children in Canada are living below the poverty line a new study indicates.The number jumps to nearly two thirds in Manitoba and Saskatchewan according to a study released Wednesday by the Canadian Centre for Policy Alternatives and Save the Children Canada.“The report’s findings that half of status First Nation children live in poverty should shock all Canadians,” said Patricia Erb, President and CEO of Save the Children, in a statement. “Looking beyond the numbers to the impact of poverty on the lives of Indigenous children explains why Save the Children is currently growing our programming in Canada.”The number drops when looking at Metis, Inuit, and non-status Indian children to 27 per cent and brings the total to nearly 40 per cent for all Indigenous children living in poverty compared to 15 per cent of non-Indigenous children.Some of the differences in child poverty appear to be where they are from. Federally funded on-reserve children faced the most hardships compared to those who lived off reserve.The federal government funds on-reserve social services, health care, education and income supports.According to the study transfer payments for these social services on-reserve have increased two per cent a year since 1996 and isn’t adjusted for population growth or need.“The removal of this cap on funding growth and an adjustment of transfers for need could reduce the alarming rate of status First Nations households living in poverty. It is a matter of choice,” said a portion of the study’s executive summary. “The failure of ongoing policies is clear. The link between the denial of basic human rights for Indigenous children and their poverty is equally clear.”The study examined child poverty statistics from the 2006 census, the most recent data available according to the authors.According to the statistics Indigenous children fall behind on family income, ability to receive an education, crowding and homelessness, poor water quality, infant mortality, health and suicide.“The Indigenous population is the fastest growing in Canada. With adequate and sustained support these people will become an integral part of society and the workforce–particularly as baby boomers retire,” said Daniel Wilson, Indigenous rights advocate and co-author of the study. “But if we refuse to address the crushing poverty facing Indigenous children, we will ensure the crisis of socioeconomic marginalization and wasted potential will continue.”Nearly $7.5 billion would be need to bring all children up to the poverty line suggested the study.news@aptn.calast_img read more

Engineering artificial cell membranes to drive in situ fibrin hydrogel formation

first_imgRe-engineering the cell membrane for improved biofunction is an emerging, powerful tool in cell biology to develop next-generation cell therapies. The process can allow users to supplement cells with added therapeutic functionalities. Additional functionalities can include cell homing, surface adhesion or resistance to hypoxia for enhanced cellular capabilities. However, the number of such examples on re-engineered plasma-membranes to activate membrane-bound enzymes that promote the assembly of extracellular matrix (ECM) proteins to promote cell functionalities are limited. 3D projection of fibrin gel containing fibrinogen stained with Alexa 594 (red) fibrinogen and hMSCs incorporating sc_thrombin [ox890] stained with Hoeschst 33342, imaged after 60 min of cell associated fibrin formation in cell culture. Credit: Nature Communications, doi: 10.1038/s41467-019-09763-0. They synthesized the artificial membrane binding thrombin complex using a two-step process to generate an active supercationic thrombin construct (sc_thrombin). Deller et al. also generated a polymer surfactant corona or green halo using electrostatic coupling of glycolic acid ethoxylate 4-nonylphenyl ether (ox890) to sc_thrombin to engineer a third variant sc_thrombin [ox890]. The scientists controlled the reaction conditions (pH, temperature and chemical composition) carefully and monitored the reaction progress using zeta potentiometry across a period of two hours. They observed the activity of thrombin by monitoring/characterizing the increasing turbidity of the fibrinogen solution. They then obtained MALDI-TOF spectra (matrix-assisted laser desorption ionization time of flight mass spectroscopy) of the native and modified thrombin to show full cationization of the construct. When Deller et al. conducted compression testing of the resultant self-supporting structures, the Young’s moduli were similar to soft fibrin hydrogels, indicating consistency. To investigate thrombin adhesion to cell membranes, the scientists chose a monolayer of bone marrow derived hMSCs (with well-characterized adipogenic, chondrogenic and osteogenic pathways). First, they incubated a monolayer of hMSCs with fluorescently-labelled analogues of thrombin; thereafter, they labeled the hMSCs with a plasma membrane-specific dye and imaged immediately to confirm the thrombin-plasma membrane binding. Using time-lapse confocal fluorescence microscopy, they showed the nucleation and fibrin growth from the cells thereafter. Welding with stem cells for next-generation surgical glues Advanced cell therapies are currently approaching clinical translation in response to an increasing demand for newly modified, cell-specific matrices (scaffolds) for biocompatible therapeutic performance. However, the rational design of matrices is extremely challenging since the cell phenotype and cell fate can be intertwined to a wide-range of scaffold-dependent factors; including cell adhesion, surrounding chemical composition, cell receptor stimulation, surface micro-/nano-morphology and mechanical stiffness. These factors immediately impact cells during in vitro tissue engineering, typically when cells are seeded to adhere on biocompatible and biodegradable scaffolds in the lab, where the scaffolds act as a surrogate extracellular matrix (ECM). Eventually, when the cells grow and differentiate, they can produce natural ECM of their own, to gradually replace the biomimetic scaffold material and form a structurally self-supporting biological entity. Deller et al. also completed cell growth assays to determine relative metabolic activities of labelled hMSCs-thrombin to show the modified cells were without observable cytotoxicity in varying concentrations of thrombin (1 µm to 25 mM). Using confocal microscopy again, the scientists showed the arrangement of fibrin structures emanating from the plasma membrane of the hMSC monolayer, in contrast to hMSCs without thrombin. The work protocol thereby generated a 3-D fibrin hydrogel construct with dense cellular aggregates surrounded by a 3-D fibrin matrix. The scientists also investigated the ability of the fibrin hydrogel system to sustain 3-D cultures for long-term growth; a requisite for tissue engineering, to show hMSC differentiation via adipogenic and osteogenic pathways. To verify the results, the scientists conducted extensive biomechanical tests on the cell types and tested for the upregulation of specific genes SOX9 and RUNX2 involved in chondrogenesis and osteogenesis respectively, using RT-PCR (reverse transcription polymerase chain reaction), to substantiate the fibrin hydrogel system sustained long-term hMSC proliferation in vitro.After confirming the membrane re-engineering approach for in lab tissue engineering applications, Deller et al. investigated the ability to produce thrombin coated cells in lab for their injection at a site of injury to initiate a healing response for tissue-engineering applications in vivo. For this, the scientists used an in vivo Zebrafish model system to perform preliminary cell transplant studies. Zebrafish is a model organism, established for fluorescently labelled live cell imaging and thrombolytic and hemostatic processes; suited for the present work. The scientists isolated, labelled and delivered fluorescently labelled primary Zebrafish fibroblasts, labelled with sc_thrombin[ox890] conjugate via microinjection to show cell survival after 3 days at a site of incisional injury. The synthesis and characterisation of the supercationic thrombin-polymer surfactant conjugate. a Schematic showing the electrostatic surface potential of native and supercationic thrombin (sc_thrombin) (PDB; 1UVS) at pH 7, highlighting the anionic (blue) and cationic (red) charged regions. Generation of the polymer surfactant corona (green halo) via electrostatic coupling of glycolic acid ethoxylate 4-nonylphenyl ether (ox890) to sc_thrombin gives [sc_thrombin][ox890]. b Zeta potential (ca. pH7; n = 3) of thrombin as a function of cationization times (0–120 min). Data reported as means ± standard deviation (s.d.). c Rate of fibrinogen solution (3.125 mg mL−1) gelation as measured by changes in turbidity (600 nm) catalysed by sc_thrombin subjected to various cationization times (0–120 min). Data shown as one-phase association curves of raw data. d MALDI-TOF MS spectra (m/z = 3) of native and sc_thrombin (60 min). Credit: Nature Communications, doi: 10.1038/s41467-019-09763-0 Schematic diagram showing in situ fibrin hydrogel formation from the membranes of bone-marrow derived human mesenchymal stem cells (hMSCs). Artificial membrane binding thrombin constructs comprising supercationic thrombin molecules (white) surrounded by a polymer surfactant corona (yellow) that associates with surface exposed cationic (red) residues spontaneously insert into bilayer regions of hMSC plasma membranes. In the presence of fibrinogen, the membrane-immobilised thrombin catalyses fibrin formation (blue fibres) within the interstitial spaces between the cells giving rise to a self-supporting hydrogel monolith. Credit: Nature Communications, doi: 10.1038/s41467-019-09763-0 More information: Robert C. Deller et al. Artificial cell membrane binding thrombin constructs drive in situ fibrin hydrogel formation, Nature Communications (2019). DOI: 10.1038/s41467-019-09763-0 D. E. Discher. Tissue Cells Feel and Respond to the Stiffness of Their Substrate, Science (2005). DOI: 10.1126/science.1116995 Tamer A.E. Ahmed et al. Fibrin: A Versatile Scaffold for Tissue Engineering Applications, Tissue Engineering Part B: Reviews (2008). DOI: 10.1089/ten.teb.2007.0435 © 2019 Science X Network In a recent study, Robert C. Deller and co-workers at the interdisciplinary departments of Cellular and Molecular Medicine, Engineering, Functional Nanomaterials and Pharmacology, and Neuroscience in the UK, bioengineered a self-contained cell matrix-forming system. In the experiments, they modified the plasma membrane of human mesenchymal stem cells (hMSCs) to integrate a new thrombin construct, which gave rise to spontaneous fibrin hydrogel nucleation and growth when supplemented with human plasma concentration levels of fibrinogen in cell culture media. The scientists bioengineered the cell membrane by synthesizing a membrane-binding supercationic thrombin-polymer surfactant complex. Thereafter, they observed cell differentiation in the resulting robust, stem cell-containing fibrin hydrogel constructs to form osteogenic and adipogenic cell lineages. The differentiated cells could eventually secrete fibrin to form self-supported bioengineered cellular monoliths that exhibited Young’s moduli as expected of the native extracellular matrix. The results are now published in Nature Communications. center_img A range of natural biocompatible polymers have produced such transient hydrogel scaffolds for tissue engineering; including chitosan, gelatin and fibrin. Fibrin hydrogels are the most popular among them, since they can be produced readily at room temperature using proteolytic cleavage. Biological fibrin formation occurs in response to injury, culminating from a biochemical cascade of proteolytic cleavage, which converts prothrombin to thrombin and forms a fibrin-hydrogel clot. Fibrin hydrogels can therefore mediate cellular biomolecular functions and regulate the osteogenic and chondrogenic differentiation of human stem cells such as hMSCs. They can also be conveniently delivered using syringes, albeit with complications related to reduced cell viability.In the present work, Deller and co-workers first described a method to synthesize supercationic thrombin-polymer surfactant complexes that spontaneously bound to the plasma membrane of hMSCs to drive in situ fibrin hydrogel nucleation and growth. The resulting self-supporting hydrogel construct allowed high levels of metabolic activity as an artificial matrix for effective differentiation of stem cells to form adipogenic or osteogenic cell lineages. The scientists then showed the feasibility of the method of cell functionalization by injecting thrombin-labelled GFP-expressing fibroblasts (GFP: Green fluorescence protein) into a zebrafish (Danio rerio) skin wound model to demonstrate their in vivo biocompatibility for hemostatic applications. Explore further Journal information: Nature Communications In vivo zebrafish injury and [sc_thrombin][ox890] labelled GFP + fibroblast addition. Schematic representation of the in vivo adult zebrafish injury model. a Wildtype (non-transgenic) recipient zebrafish were anaesthetized and a 4 mm incisional injury made on the ventral upper thorax. A lateral view is shown. b Unlabelled or [sc_thrombin][ox890] labelled, FACS sorted GFP+ fibroblasts were injected at six sites around the edge of the incisional injury. At the desired time-point, fish were sacrificed and the tissue surrounding the incision was fixed, imaged and embedded for sectioning. A ventral view is shown. Ventral view of the area of tissue surrounding the incision at 3 dpi following transfer of c unlabelled or d [sc_thrombin][ox890] labelled GFP+ fibroblasts. Similar numbers of cells were retained at all wounds. The red line depicts the approximate position of the incisional injury which is fully re-epithelialized at this stage. Sections through the injury region at 3 dpi following transfer of e unlabelled or f [sc_thrombin][ox890] labelled GFP+ fibroblasts. No obvious differences were observed between wounds containing labelled or unlabelled cells. Arrows indicate the position of the incision. Credit: Nature Communications, doi: 10.1038/s41467-019-09763-0. Citation: Engineering artificial cell membranes to drive in situ fibrin hydrogel formation (2019, May 6) retrieved 18 August 2019 from https://phys.org/news/2019-05-artificial-cell-membranes-situ-fibrin.html Using macroscopic observations and histological assays, the scientists further revealed that there were no adverse effects between fish injected with engineered or native fibroblasts. However, Deller et al. expect to complete further investigations to understand the precise effects on the specific wound healing response using bioengineered cells in the future.In this way, Deller et al. synthesized and characterized a new membrane active thrombin construct and demonstrated its application to drive in situ fibrin formation in the plasma membranes of stem cells. The scientists showed that thrombin-based fibrin hydrogel constructs prepared using the new protocol supported high levels of cell growth and viability to eventually produce a self-supporting tissue engineered construct. The stem cells were also able to differentiate along the well-defined adipogenic and osteogenic pathways while demonstrating Young’s moduli similar to the native cells to indicate high levels of integration of the modifications. Deller et al. propose to optimize the protocols for further experiments in vitro prior to in vivo translation, to gain further insight to the enzymatic activity of cell membrane bound bioengineered proteins to develop biocompatible, hemostatic products. Evaluating rhodamine (rh) labelled rh_thrombin, rh_sc_thrombin and [rh_sc_thrombin][ox890] on hMSC plasma membrane affinity. Cells labelled with CellMask (green) and rhodamine labelled thrombin (magenta) visualized with confocal microscopy. Video shows the rh_sc_thrombin [ox890] labelled hMSCs supplemented with fibrin gel conjugating with fibrinogen (green) to highlight fibrin formation emanating from the bioengineered cell surface. Credit: Nature Communications, doi: 10.1038/s41467-019-09763-0 , Science This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.last_img read more