October 28, 2018 Cannabis oil explosion leaves one seriously injured in South Bay KUSI Newsroom, Posted: October 28, 2018 SAN DIEGO (KUSI) — A person was seriously injured in an explosion during an apparent cannabis oil extraction operation at a South Bay home, an officer said Sunday.Police and paramedics were called around 7:30 p.m. Saturday evening to a house on Leider Drive near Green Bay Street, in Egger Highlands, according to San Diego police Officer Sarah Foster.One person was badly burned and had to be taken to a hospital, Foster said.No identifying information was available on the person. KUSI Newsroom Updated: 7:57 PM Categories: Local San Diego News FacebookTwitter
Publishers Press/Publishers Printing Company Label-Pak Division has been awarded the Flexographic Technical Association’s (FTA) Gold Award, as well as the Best of Show Award for Narrow Web Publication for Alpinist Magazine’s “Autumn 2010” cover.The Label-Pak Division was awarded one of six Best of Show Awards given annually. Over 900 submissions were entered for consideration.Other Narrow Web Gold Publication winners include MPI Label Systems’ Bushdoctor Microbe Brew (label) and Labeltronix’s NLN Chocolate Banana Lean Pro Matrix and Eagle Castle 2009 Chardonnay (both labels).The 145-year-old Publishers Press/Publishers Printing Company is based in Louisville, Kentucky. The company currently offers magazine, catalogue and full service commercial printing, distribution, email marketing and label printing.
A special exclusive ticket presale for Citi card holders will run from Tuesday, April 10, at noon local time through Thursday, April 12 at 10 p.m. local time. Tickets for the general public will go on sale on April 13 at 10 a.m. local time via LiveNation.Catching Up On Music News Powered By The Recording Academy Just Got Easier. Have A Google Home Device? “Talk To GRAMMYs”Read more The Offspring, 311 Plot 2018 Arena Tour News Email Facebook Twitter The legends of ’90s alternative radio will be joined by special guests Gym Class HeroesBrian HaackGRAMMYs Apr 9, 2018 – 4:00 pm Summer 2018 is shaping up to be a bit of a victory lap for ’90s alternative, hard rock and skate punk bands and their fans. Already announced are the anticipated return of System Of A Down, a three-day all-punk campout festival hosted by NoFX’s Fat Mike, and a unique “tri-headlining” tour featuring Stone Temple Pilots, Bush and Cult making for an increasingly packed summer festival and tour schedule. Now added to mix is a brand new co-headlining tour featuring proto-pop punk hitmakers 311 and the Offspring, joined by special guests Gym Class Heroes.The “Never-Ending Summer Tour” will hopscotch through 29 cities across North America for a series of area shows and festival appearances kicking off on July 25 at Mountain View, Calif., Shoreline Amphitheater and run through Sept. 9, where the tour will close out in Wichita, Kan. The Offspring And 311 Plot 2018 Arena Tour offspring-311-plot-2018-arena-tour
– / 5Please Sir, I want some more.Even for people unfamiliar with the story of Oliver Twist, many still know that famous line.Now, imagine someone saying it in sign language.Recently, Theatre Under the Stars gave a performance of the musical Oliver!. But on this night at the Hobby Center, there aren’t just actors onstage. On the floor to the right, in the dimly-lit performance hall, there’s another spotlight on two people dressed in black and gray, acting out the scenes in American Sign Language, or ASL.A musical for people who can’t hear? It may seem contradictory at first.“Deaf people get music,” says the Hobby Center’s Audience Services Manager Judi Stallings. “Just because they can’t hear everything, it doesn’t mean they don’t understand it. They get the rhythm, the flow, the fluidity of the music.”The Hobby Center is partnering with TUTS and the University of Houston’s American Sign Language Interpreting program to make the arts more accessible to Houston’s deaf community. It’s the first time they’re trying something like this.Brittany Best is majoring in American Sign Language Interpreting at UH and is one of the seven seniors responsible for translating the lines of the 58 characters in Oliver!. It’s pretty demanding.“I’ve got to know the actors’ lines; I’ve got to know my interpretation of those lines; and I’ve got to know what they’re doing onstage, because I’m not looking at them,” Best says. “So I’ve got to know all three and keep in line with the music (and) the tempo of the song.”The preparation began weeks in advance.“They engaged in complete script analysis and, along with me, worked through the script line, by line, by line,” says Sharon Hill, Program Coordinator and faculty member for UH’s ASLI program. She’s been instrumental in getting the project started.ASL is its own language and doesn’t have the same sentence structure as English, which means that translating word for word doesn’t always work. That’s also why subtitles aren’t usually ideal when trying to translate dialogue and music.“As Long as He Needs Me is really a challenging song,” says UH senior and ASL interpreter Barae Frizzel. “I almost spent a day just trying to study that piece. (There are) no signs that you can match to it. It’s more of a feeling and emotion that you have to connect with your audience.”When the lights come up at the end of the show, some people up front are waving their hands in the air. (Think jazz hands, but with more excitement.) That’s the deaf version of applause.Kristina Rodriguez, another student who was signing in the performance, has a unique experience with sign language. Both her parents are deaf and she grew up speaking ASL. This was her mom’s first time to a TUTS show.When she was asked what it was like to see her daughter in a live performance.Kristina translated the question and answer: “I’ve never seen my daughter act that way and have so much expression on her face. It was really exciting to watch. I’m just really happy to see her here.”It was also the first time for Robyn Brittan, another member of the deaf community.Sharon Hill translated her comment: “I see, as a deaf person, that I have access in my language. I miss not one piece of the show.”Stallings says this is a test-run to see if it’s something the Hobby Center can begin to offer more often as part of their accessibility initiative they began this year.“People that I know in the deaf community have assured me, ‘Don’t leave me out just because you think I can’t hear the music,’” she says. “There’s still something there for them.” 00:00 /04:01 Listen X To embed this piece of audio in your site, please use this code: Share
The Asus Eee Top comes in two versions with the ET1602 and the ET1603. Inside the Asus Eee Top ET1603 a discrete ATI Mobility Radeon HD3450 is responsible for more graphics power, as compared to the ET1602, which enables the system to run graphically-intensive 3D applications and games more fluidly. Even though the Asus Eee all-in-one desktop has touch-sensitive screen, it still comes bundled with keyboard and mouse.Setting up the Eee Top is as easy as taking it out of the box and plugging it in. This system is also the perfect computer for beginners. It features an exclusive Easy Mode within Windows that makes commonly-used applications easily accessible. With its sharp and vibrant widescreen (16:9) LCD, the Eee Top becomes the perfect Media Entertainment Center. Asus´s patient Splendid Video Intelligence Technology renders the video display with a vivid and clear image regardless of environmental lighting and SRS technology works in tandem with the Eee Top´s built-in high fidelity speakers to deliver crystal clear audio.The Eee Top touchscreen PC is first to ship in Taiwan, Hong Kong and the UK. In 2009 Asus is also adding larger screen models with 20- and 22-inch Asus Eee Top PCs. There is no information yet as to when these PC´s will be available in the US. Mini-PC-Pro Reviews ASUS Eee Top PC in a live demonstration. © 2008 PhysOrg.com (PhysOrg.com) — Asus has just revealed their 15.6-inch Eee Top touchscreen PC that is powered by an Intel Atom processor N270 (1.60GHz) and Intel 945 GSE core-logic with built-in graphics core. The system features a 160GB hard drive, 1GB of RAM, 4-in-1 card reader, integrated camera, Wi-Fi 802.11n controller and Windows XP Home operating system. Explore further Cute, efficient Eee Top looks like future of home computing Citation: ASUS Eee Top Touchscreen PC’s Coming 1H 2009 (2008, November 25) retrieved 18 August 2019 from https://phys.org/news/2008-11-asus-eee-touchscreen-pc-1h.html This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.
Re-engineering the cell membrane for improved biofunction is an emerging, powerful tool in cell biology to develop next-generation cell therapies. The process can allow users to supplement cells with added therapeutic functionalities. Additional functionalities can include cell homing, surface adhesion or resistance to hypoxia for enhanced cellular capabilities. However, the number of such examples on re-engineered plasma-membranes to activate membrane-bound enzymes that promote the assembly of extracellular matrix (ECM) proteins to promote cell functionalities are limited. 3D projection of fibrin gel containing fibrinogen stained with Alexa 594 (red) fibrinogen and hMSCs incorporating sc_thrombin [ox890] stained with Hoeschst 33342, imaged after 60 min of cell associated fibrin formation in cell culture. Credit: Nature Communications, doi: 10.1038/s41467-019-09763-0. They synthesized the artificial membrane binding thrombin complex using a two-step process to generate an active supercationic thrombin construct (sc_thrombin). Deller et al. also generated a polymer surfactant corona or green halo using electrostatic coupling of glycolic acid ethoxylate 4-nonylphenyl ether (ox890) to sc_thrombin to engineer a third variant sc_thrombin [ox890]. The scientists controlled the reaction conditions (pH, temperature and chemical composition) carefully and monitored the reaction progress using zeta potentiometry across a period of two hours. They observed the activity of thrombin by monitoring/characterizing the increasing turbidity of the fibrinogen solution. They then obtained MALDI-TOF spectra (matrix-assisted laser desorption ionization time of flight mass spectroscopy) of the native and modified thrombin to show full cationization of the construct. When Deller et al. conducted compression testing of the resultant self-supporting structures, the Young’s moduli were similar to soft fibrin hydrogels, indicating consistency. To investigate thrombin adhesion to cell membranes, the scientists chose a monolayer of bone marrow derived hMSCs (with well-characterized adipogenic, chondrogenic and osteogenic pathways). First, they incubated a monolayer of hMSCs with fluorescently-labelled analogues of thrombin; thereafter, they labeled the hMSCs with a plasma membrane-specific dye and imaged immediately to confirm the thrombin-plasma membrane binding. Using time-lapse confocal fluorescence microscopy, they showed the nucleation and fibrin growth from the cells thereafter. Welding with stem cells for next-generation surgical glues Advanced cell therapies are currently approaching clinical translation in response to an increasing demand for newly modified, cell-specific matrices (scaffolds) for biocompatible therapeutic performance. However, the rational design of matrices is extremely challenging since the cell phenotype and cell fate can be intertwined to a wide-range of scaffold-dependent factors; including cell adhesion, surrounding chemical composition, cell receptor stimulation, surface micro-/nano-morphology and mechanical stiffness. These factors immediately impact cells during in vitro tissue engineering, typically when cells are seeded to adhere on biocompatible and biodegradable scaffolds in the lab, where the scaffolds act as a surrogate extracellular matrix (ECM). Eventually, when the cells grow and differentiate, they can produce natural ECM of their own, to gradually replace the biomimetic scaffold material and form a structurally self-supporting biological entity. Deller et al. also completed cell growth assays to determine relative metabolic activities of labelled hMSCs-thrombin to show the modified cells were without observable cytotoxicity in varying concentrations of thrombin (1 µm to 25 mM). Using confocal microscopy again, the scientists showed the arrangement of fibrin structures emanating from the plasma membrane of the hMSC monolayer, in contrast to hMSCs without thrombin. The work protocol thereby generated a 3-D fibrin hydrogel construct with dense cellular aggregates surrounded by a 3-D fibrin matrix. The scientists also investigated the ability of the fibrin hydrogel system to sustain 3-D cultures for long-term growth; a requisite for tissue engineering, to show hMSC differentiation via adipogenic and osteogenic pathways. To verify the results, the scientists conducted extensive biomechanical tests on the cell types and tested for the upregulation of specific genes SOX9 and RUNX2 involved in chondrogenesis and osteogenesis respectively, using RT-PCR (reverse transcription polymerase chain reaction), to substantiate the fibrin hydrogel system sustained long-term hMSC proliferation in vitro.After confirming the membrane re-engineering approach for in lab tissue engineering applications, Deller et al. investigated the ability to produce thrombin coated cells in lab for their injection at a site of injury to initiate a healing response for tissue-engineering applications in vivo. For this, the scientists used an in vivo Zebrafish model system to perform preliminary cell transplant studies. Zebrafish is a model organism, established for fluorescently labelled live cell imaging and thrombolytic and hemostatic processes; suited for the present work. The scientists isolated, labelled and delivered fluorescently labelled primary Zebrafish fibroblasts, labelled with sc_thrombin[ox890] conjugate via microinjection to show cell survival after 3 days at a site of incisional injury. The synthesis and characterisation of the supercationic thrombin-polymer surfactant conjugate. a Schematic showing the electrostatic surface potential of native and supercationic thrombin (sc_thrombin) (PDB; 1UVS) at pH 7, highlighting the anionic (blue) and cationic (red) charged regions. Generation of the polymer surfactant corona (green halo) via electrostatic coupling of glycolic acid ethoxylate 4-nonylphenyl ether (ox890) to sc_thrombin gives [sc_thrombin][ox890]. b Zeta potential (ca. pH7; n = 3) of thrombin as a function of cationization times (0–120 min). Data reported as means ± standard deviation (s.d.). c Rate of fibrinogen solution (3.125 mg mL−1) gelation as measured by changes in turbidity (600 nm) catalysed by sc_thrombin subjected to various cationization times (0–120 min). Data shown as one-phase association curves of raw data. d MALDI-TOF MS spectra (m/z = 3) of native and sc_thrombin (60 min). Credit: Nature Communications, doi: 10.1038/s41467-019-09763-0 Schematic diagram showing in situ fibrin hydrogel formation from the membranes of bone-marrow derived human mesenchymal stem cells (hMSCs). Artificial membrane binding thrombin constructs comprising supercationic thrombin molecules (white) surrounded by a polymer surfactant corona (yellow) that associates with surface exposed cationic (red) residues spontaneously insert into bilayer regions of hMSC plasma membranes. In the presence of fibrinogen, the membrane-immobilised thrombin catalyses fibrin formation (blue fibres) within the interstitial spaces between the cells giving rise to a self-supporting hydrogel monolith. Credit: Nature Communications, doi: 10.1038/s41467-019-09763-0 More information: Robert C. Deller et al. Artificial cell membrane binding thrombin constructs drive in situ fibrin hydrogel formation, Nature Communications (2019). DOI: 10.1038/s41467-019-09763-0 D. E. Discher. Tissue Cells Feel and Respond to the Stiffness of Their Substrate, Science (2005). DOI: 10.1126/science.1116995 Tamer A.E. Ahmed et al. Fibrin: A Versatile Scaffold for Tissue Engineering Applications, Tissue Engineering Part B: Reviews (2008). DOI: 10.1089/ten.teb.2007.0435 © 2019 Science X Network In a recent study, Robert C. Deller and co-workers at the interdisciplinary departments of Cellular and Molecular Medicine, Engineering, Functional Nanomaterials and Pharmacology, and Neuroscience in the UK, bioengineered a self-contained cell matrix-forming system. In the experiments, they modified the plasma membrane of human mesenchymal stem cells (hMSCs) to integrate a new thrombin construct, which gave rise to spontaneous fibrin hydrogel nucleation and growth when supplemented with human plasma concentration levels of fibrinogen in cell culture media. The scientists bioengineered the cell membrane by synthesizing a membrane-binding supercationic thrombin-polymer surfactant complex. Thereafter, they observed cell differentiation in the resulting robust, stem cell-containing fibrin hydrogel constructs to form osteogenic and adipogenic cell lineages. The differentiated cells could eventually secrete fibrin to form self-supported bioengineered cellular monoliths that exhibited Young’s moduli as expected of the native extracellular matrix. The results are now published in Nature Communications. A range of natural biocompatible polymers have produced such transient hydrogel scaffolds for tissue engineering; including chitosan, gelatin and fibrin. Fibrin hydrogels are the most popular among them, since they can be produced readily at room temperature using proteolytic cleavage. Biological fibrin formation occurs in response to injury, culminating from a biochemical cascade of proteolytic cleavage, which converts prothrombin to thrombin and forms a fibrin-hydrogel clot. Fibrin hydrogels can therefore mediate cellular biomolecular functions and regulate the osteogenic and chondrogenic differentiation of human stem cells such as hMSCs. They can also be conveniently delivered using syringes, albeit with complications related to reduced cell viability.In the present work, Deller and co-workers first described a method to synthesize supercationic thrombin-polymer surfactant complexes that spontaneously bound to the plasma membrane of hMSCs to drive in situ fibrin hydrogel nucleation and growth. The resulting self-supporting hydrogel construct allowed high levels of metabolic activity as an artificial matrix for effective differentiation of stem cells to form adipogenic or osteogenic cell lineages. The scientists then showed the feasibility of the method of cell functionalization by injecting thrombin-labelled GFP-expressing fibroblasts (GFP: Green fluorescence protein) into a zebrafish (Danio rerio) skin wound model to demonstrate their in vivo biocompatibility for hemostatic applications. Explore further Journal information: Nature Communications In vivo zebrafish injury and [sc_thrombin][ox890] labelled GFP + fibroblast addition. Schematic representation of the in vivo adult zebrafish injury model. a Wildtype (non-transgenic) recipient zebrafish were anaesthetized and a 4 mm incisional injury made on the ventral upper thorax. A lateral view is shown. b Unlabelled or [sc_thrombin][ox890] labelled, FACS sorted GFP+ fibroblasts were injected at six sites around the edge of the incisional injury. At the desired time-point, fish were sacrificed and the tissue surrounding the incision was fixed, imaged and embedded for sectioning. A ventral view is shown. Ventral view of the area of tissue surrounding the incision at 3 dpi following transfer of c unlabelled or d [sc_thrombin][ox890] labelled GFP+ fibroblasts. Similar numbers of cells were retained at all wounds. The red line depicts the approximate position of the incisional injury which is fully re-epithelialized at this stage. Sections through the injury region at 3 dpi following transfer of e unlabelled or f [sc_thrombin][ox890] labelled GFP+ fibroblasts. No obvious differences were observed between wounds containing labelled or unlabelled cells. Arrows indicate the position of the incision. Credit: Nature Communications, doi: 10.1038/s41467-019-09763-0. Citation: Engineering artificial cell membranes to drive in situ fibrin hydrogel formation (2019, May 6) retrieved 18 August 2019 from https://phys.org/news/2019-05-artificial-cell-membranes-situ-fibrin.html Using macroscopic observations and histological assays, the scientists further revealed that there were no adverse effects between fish injected with engineered or native fibroblasts. However, Deller et al. expect to complete further investigations to understand the precise effects on the specific wound healing response using bioengineered cells in the future.In this way, Deller et al. synthesized and characterized a new membrane active thrombin construct and demonstrated its application to drive in situ fibrin formation in the plasma membranes of stem cells. The scientists showed that thrombin-based fibrin hydrogel constructs prepared using the new protocol supported high levels of cell growth and viability to eventually produce a self-supporting tissue engineered construct. The stem cells were also able to differentiate along the well-defined adipogenic and osteogenic pathways while demonstrating Young’s moduli similar to the native cells to indicate high levels of integration of the modifications. Deller et al. propose to optimize the protocols for further experiments in vitro prior to in vivo translation, to gain further insight to the enzymatic activity of cell membrane bound bioengineered proteins to develop biocompatible, hemostatic products. Evaluating rhodamine (rh) labelled rh_thrombin, rh_sc_thrombin and [rh_sc_thrombin][ox890] on hMSC plasma membrane affinity. Cells labelled with CellMask (green) and rhodamine labelled thrombin (magenta) visualized with confocal microscopy. Video shows the rh_sc_thrombin [ox890] labelled hMSCs supplemented with fibrin gel conjugating with fibrinogen (green) to highlight fibrin formation emanating from the bioengineered cell surface. Credit: Nature Communications, doi: 10.1038/s41467-019-09763-0 , Science This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.