Sampdoria president Ferrero insists no-one will be soldby Carlos Volcano10 months agoSend to a friendShare the loveSampdoria president Massimo Ferrero insists no-one will be sold this month.Samp are just three points off the top four.“After the game, on the bus, I congratulated all the players,” Ferrero told Il Secolo XIX.“I told them I was proud of how they face the Bianconeri. The fans can be calm, I won’t sell anyone. The base of the group is strong and united, it will remain.“At the most we’ll see if there’s an opportunity to bring in someone promising, like in recent years.” TagsTransfersAbout the authorCarlos VolcanoShare the loveHave your say
zoom The Marshall Islands-based holding company Advantage Tankers has allegedly bought five MR1 tankers from Cyprus-based shipping company Interorient, VesselsValue’s data shows.The ships in question are Baltic Marshall, Baltic Merchant, Baltic Ambition, Baltic Faith and Baltic Force. They were reportedly sold in an en bloc transaction for USD 12 million each on May 12.All of the five chemical/product tankers fly the flag of Hong Kong and were built at South Korean Hyundai Mipo shipyard in 2006.With a deadweight of around 37,000 tons, each of the ships has a length of 183 meters and a width of 27 meters.World Maritime News contacted Advantage Tankers and Interorient for more details on the matter, however, the companies are yet to reply.With the newest addition, Advantage Tankers makes an entry into the product tanker segment. Currently, the company has a fleet of eleven tankers comprising five Aframaxes and six Suezmaxes.World Maritime News Staff
Bayer Leverkusen has sold goalkeeper Bernd Leno to Arsenal for around €19 million, completing Unai Emery’s second major signing as manager.The 26-year-old German international Leno has arrived on a contract that should keep him at the Emirates until 2023, making him the long-term replacement for current number one Petr Cech, reports Four-Four-Two. Emery was delighted after unveiling his latest acquisition, saying: “We are very pleased that Bernd Leno will be joining us,”“Bernd is a goalkeeper of high quality and experience. We are all excited that Bernd has chosen Arsenal and look forward to start working with him in pre-season.” Merson believes Arsenal should sign Sancho Manuel R. Medina – September 14, 2019 Borussia Dortmund winger Jadon Sancho might be the perfect player to play for the Gunners, according to former England international Paul Merson.Welcome to Arsenal, @Bernd_Leno – we’re delighted to have you here ?#HeyLeno pic.twitter.com/m4yT22I58V— Arsenal FC (@Arsenal) June 19, 2018Leno was a major part of the Leverkusen squad that achieved a 5th place finish in the Bundesliga last year and he has won 6 caps for his country to date – though unfortunately, he did not make Joachim Lowes final squad for the World Cup.After the purchase of Stephan Lichtsteiner last month, Emery has managed to further enhance his options defensively with the signing of Leno, as his new look Arsenal side begins to take shape.
Arsenal midfielder Lucas Torreira has revealed he received a surprise phone call from Unai Emery during the World Cup in Russia.Emery, who was appointed manager of the Gunners at the end of last season phoned Torreira to personally persuade him to move to Arsenal.The Uruguay international confessed they were a host of teams interested in his services during the summer transfer window but he never thought he would move to England.However, one phone call from the former Paris Saint-Germain boss changed everything.Following his country’s exit from the World Cup the North London club didn’t waste any time signing the midfielder, securing him on a £22million deal.Premier League Betting: Match-day 5 Stuart Heath – September 14, 2019 Going into the Premier League’s match-day five with a gap already beginning to form at the top of the league. We will take a…“At the end of my last season at Sampdoria there were lots of teams being talked about,” Torreira told Arsenal media as quoted in the Mirror.“I never imagined coming here to England because it was mainly Italian clubs being mentioned.”“Before I left for the World cup I got a call from the coach which was a massive surprise for me. I knew that I had earned it because of all my hard work and everything that I had done. That phone call made me a happy man.”“It allowed me to relax too because it allowed me to focus all my attention on the national team and the World Cup. I’m so proud to be part of such an important team.”
Manager Gennaro Gattuso believes that Suso deserves a contract extension at AC Milan based on his contributions to the team.Since arriving from a lacklustre spell at Liverpool in January 2015, Suso has emerged as one of the most exciting midfielders in the Serie A.In light of this, the four-time Spain international had been linked with a return to Liverpool along with a switch to Tottenham in the Premier League last season.Suso is contracted at Milan until June 2022, but has continued to be of great interest to rival clubs with reports stating that he’s got a €40m release clause.And now Gattuso hopes the Rossoneri board will award his star playmaker with a deserved new contract.“My job is to coach, I am not an agent or a sports director. He deserves a contract renewal, but it depends on the management,” said Gattuso on Twitter.“There is great respect for Suso and he is contributing a lot to the team.Lukaku backed to beat Ronaldo in Serie A scoring charts Andrew Smyth – September 14, 2019 Former Inter Milan star Andy van der Meyde is confident Romelu Lukaku will outscore Cristiano Ronaldo in this season’s Serie A.“He immediately made himself available after coming back from a leg injury [in January].”Suso has managed six goals and eight assists in 26 appearances across all competitions for Milan this season.Gattuso’s side will now turn their attention towards Saturday’s visit to Atalanta in their Serie A match, where they hope to grab victory to hold on to fourth-place in the table.And the Milan coach warned his players that nobody is “untouchable” when it comes to a spot in his starting line-up.“We are doing well and we must keep this up,” said Gattuso.“I don’t like to talk about untouchables, as every player must fight for their place. Important teams are born out of respect, I like the fact that different players are starting to take responsibility both on and off the pitch.The Atalanta and Milan game will begin at 8:30 PM (CET) on Saturday.MILAN, ITALY – FEBRUARY 10: Krzysztof Piatek (2nd L) of AC Milan celebrates his goal with his team-mates during the Serie A match between AC Milan and Cagliari at Stadio Giuseppe Meazza on February 10, 2019 in Milan, Italy. (Photo by Marco Luzzani/Getty Images)
Manuel Pellegrini is confident that youngster Declan Rice will have a bright future after he netted his first goal for West Ham.Rice who turns 20 on Monday, has mustered 50 first team appearances for the Hammers and bagged the winning goal against Arsenal.The youngster extended his deal through to 2024 and revealed that he will make a call on his international future soon after meetings with Gareth Southgate and his Republic of Ireland counterpart Mick McCarthy.“I am sure that Declan will be a top player,” Pellegrini disclosed via the Evening Standard.Rice tips Mount for greater things Taimoor Khan – September 11, 2019 Declan Rice says that Mason Mount is going to match the exploits of Frank Lampard when he was in his prime during his playing…“He is improving in every game that he plays and is now more used to give us a good build up in front and not so many passes that before he played back.“He is very strong in aerial duels, strong in 1v1 and has a good technique which he demonstrated today in the goal he scored so I am sure that we are seeing a top player soon.“Not only for West Ham but also for the national squad he decides to play.”
October 28, 2018 Cannabis oil explosion leaves one seriously injured in South Bay KUSI Newsroom, Posted: October 28, 2018 SAN DIEGO (KUSI) — A person was seriously injured in an explosion during an apparent cannabis oil extraction operation at a South Bay home, an officer said Sunday.Police and paramedics were called around 7:30 p.m. Saturday evening to a house on Leider Drive near Green Bay Street, in Egger Highlands, according to San Diego police Officer Sarah Foster.One person was badly burned and had to be taken to a hospital, Foster said.No identifying information was available on the person. KUSI Newsroom Updated: 7:57 PM Categories: Local San Diego News FacebookTwitter
The rock python is “vicious”, according to Kenneth Krysko of the Florida Museum of Natural History. It is even meaner than the Burmese pythons, which have been known to eat alligators, and which have already taken up residence in the nearby Everglades National Park, where they have wiped out thousands of native animals. Another major alien in the park is the boa constrictor.Six specimens of Africa’s longest snake have been discovered in a single square mile west of Miami, Florida, since the first was sighted in 2002. Krysko believes the original snakes may have been released by pet breeders when they grew too big, or when the breeders were surprised by their aggressiveness. The finding of two hatchlings and a pregnant female suggests the snakes are settling in.The rock python, like the Burmese python and boa constrictor, is not poisonous but kills its prey by constricting it and suffocating it to death before swallowing it.With the Everglades National Park just across the road, the rock python may already have joined the other giant aliens in the park in wiping out the native fauna. According to Robert Reed of the U.S. Geological Survey in Colorado, the new snake might eat any warm-blooded animal it can ingest. The Burmese python is known to eat dozens of species, including white-tailed deer and even six-foot alligators, and Reed expects the rock python to do the same.Another potential problem is inter-breeding of the African rock python and the Burmese python to produce a hybrid species. This has been known to happen in captivity, and while most such offspring are infertile, there is a possibility that offspring of the two species could be even hardier and more deadly than their parents, according to Reed.Kristina Serbesoff-King of the Nature Conservancy in Florida said that we should learn from past mistakes and try to eliminate the rock python while it is still confined to a relatively small area. One approach could be a python patrol, similar to the one the Nature Conservancy ran to try to reduce the numbers of Burmese pythons.If the African rock python is not stopped in its tracks, the scientists agree the problem may be even worse than the Burmese python problem because the rock python is far more aggressive. In its native home the African rock python is known to attack humans, according to Krysko, and if hidden in a swamp, it could strike before you even knew it was there. It could pose a particular danger for pets and small children.© 2009 PhysOrg.com (PhysOrg.com) — New studies suggest a 20 foot snake, the African rock python, is making its home in Florida and could soon invade the Everglades National Park. Python molurus bivittatus. Image: Wikipedia. Explore further Citation: ‘Vicious’ Giant Python Invading Florida (2009, September 21) retrieved 18 August 2019 from https://phys.org/news/2009-09-vicious-giant-python-invading-florida.html Fla. governor approves plan to capture, kill Burmese pythons This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.
Play Movie of nanoparticles moving through mucus. Credit: Schneider et al. Sci. Adv. 2017;3:e1601556 The nanoparticles, the team notes, were created using biodegradable materials that prior research found safe for internal use. Testing in mice, they report, showed the particle carriers stayed in the lungs for several hours—they also proved to be more effective than conventional therapies at reducing asthma symptoms such as irritation. The researchers suggest that MPPs could offer a better treatment plan for lung patients by providing a therapy that maintains drug levels in the lungs for longer periods of time, reducing the need for repeated dosing, which itself can cause lung irritation. PausePlay% buffered00:0000:00UnmuteMuteDisable captionsEnable captionsSettingsCaptionsDisabledQuality0SpeedNormalCaptionsGo back to previous menuQualityGo back to previous menuSpeedGo back to previous menu0.5×0.75×Normal1.25×1.5×1.75×2×Exit fullscreenEnter fullscreen More information: Craig S. Schneider et al. Nanoparticles that do not adhere to mucus provide uniform and long-lasting drug delivery to airways following inhalation, Science Advances (2017). DOI: 10.1126/sciadv.1601556AbstractMucoadhesive particles (MAP) have been widely explored for pulmonary drug delivery because of their perceived benefits in improving particle residence in the lungs. However, retention of particles adhesively trapped in airway mucus may be limited by physiologic mucus clearance mechanisms. In contrast, particles that avoid mucoadhesion and have diameters smaller than mucus mesh spacings rapidly penetrate mucus layers [mucus-penetrating particles (MPP)], which we hypothesized would provide prolonged lung retention compared to MAP. We compared in vivo behaviors of variously sized, polystyrene-based MAP and MPP in the lungs following inhalation. MAP, regardless of particle size, were aggregated and poorly distributed throughout the airways, leading to rapid clearance from the lungs. Conversely, MPP as large as 300 nm exhibited uniform distribution and markedly enhanced retention compared to size-matched MAP. On the basis of these findings, we formulated biodegradable MPP (b-MPP) with an average diameter of <300 nm and examined their behavior following inhalation relative to similarly sized biodegradable MAP (b-MAP). Although b-MPP diffused rapidly through human airway mucus ex vivo, b-MAP did not. Rapid b-MPP movements in mucus ex vivo correlated to a more uniform distribution within the airways and enhanced lung retention time as compared to b-MAP. Furthermore, inhalation of b-MPP loaded with dexamethasone sodium phosphate (DP) significantly reduced inflammation in a mouse model of acute lung inflammation compared to both carrier-free DP and DP-loaded MAP. These studies provide a careful head-to-head comparison of MAP versus MPP following inhalation and challenge a long-standing dogma that favored the use of MAP for pulmonary drug delivery. Play Movie of nanoparticles moving through mucus. Credit: Schneider et al. Sci. Adv. 2017;3:e1601556 Citation: Nanoparticles pass through mucus membranes in lungs to deliver pulmonary drugs (2017, April 6) retrieved 18 August 2019 from https://phys.org/news/2017-04-nanoparticles-mucus-membranes-lungs-pulmonary.html Journal information: Science Advances Lung problems impact the lives of millions of people. Such conditions include asthma, in which bronchi spasm, making it difficult to breathe; cystic fibrosis, in which over-production of mucus leads to blocking of bronchi; and COPD, in which obstructions form in bronchial passages. Fortunately, these types of ailments are all treatable to some degree, though they cannot be cured. For that reason, scientists continue to look for ways to improve current therapies.Currently, lung ailments such as cystic fibrosis, COPD and asthma are treated with inhaled drugs such as corticosteroids that adhere to the walls of air passages. In some instances, they are carried by what are known as mucoadhesive particles, (MAPs), but, as the researchers note, thick mucus often builds up on such passageways, lessening the effectiveness of the delivery system. In this new effort, the researchers took a different approach—rather than trying to make medicines that adhere do their job better, they turned to nanoparticles that are small enough to make their way through mucus membranes to the lining of the lungs themselves, offering direct medication application to affected areas. Called mucus-penetrating particles (MPP), they remain in the lungs, releasing medication for an extended period of time. The making of mucus in common lung diseases PausePlay% buffered00:0000:00UnmuteMuteDisable captionsEnable captionsSettingsCaptionsDisabledQuality0SpeedNormalCaptionsGo back to previous menuQualityGo back to previous menuSpeedGo back to previous menu0.5×0.75×Normal1.25×1.5×1.75×2×Exit fullscreenEnter fullscreen This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only. © 2017 Phys.org Explore further (Phys.org)—A team of researchers from multiple institutions in the U.S. has developed a new way to treat lung disease—using nanoparticles to transport chemicals through the thick mucus membranes that can coat pulmonary airways. In their paper published in the journal Science Advances, the team describes how they used particles small enough to move through holes in the mesh that makes up mucus to deliver helpful drugs.
Re-engineering the cell membrane for improved biofunction is an emerging, powerful tool in cell biology to develop next-generation cell therapies. The process can allow users to supplement cells with added therapeutic functionalities. Additional functionalities can include cell homing, surface adhesion or resistance to hypoxia for enhanced cellular capabilities. However, the number of such examples on re-engineered plasma-membranes to activate membrane-bound enzymes that promote the assembly of extracellular matrix (ECM) proteins to promote cell functionalities are limited. 3D projection of fibrin gel containing fibrinogen stained with Alexa 594 (red) fibrinogen and hMSCs incorporating sc_thrombin [ox890] stained with Hoeschst 33342, imaged after 60 min of cell associated fibrin formation in cell culture. Credit: Nature Communications, doi: 10.1038/s41467-019-09763-0. They synthesized the artificial membrane binding thrombin complex using a two-step process to generate an active supercationic thrombin construct (sc_thrombin). Deller et al. also generated a polymer surfactant corona or green halo using electrostatic coupling of glycolic acid ethoxylate 4-nonylphenyl ether (ox890) to sc_thrombin to engineer a third variant sc_thrombin [ox890]. The scientists controlled the reaction conditions (pH, temperature and chemical composition) carefully and monitored the reaction progress using zeta potentiometry across a period of two hours. They observed the activity of thrombin by monitoring/characterizing the increasing turbidity of the fibrinogen solution. They then obtained MALDI-TOF spectra (matrix-assisted laser desorption ionization time of flight mass spectroscopy) of the native and modified thrombin to show full cationization of the construct. When Deller et al. conducted compression testing of the resultant self-supporting structures, the Young’s moduli were similar to soft fibrin hydrogels, indicating consistency. To investigate thrombin adhesion to cell membranes, the scientists chose a monolayer of bone marrow derived hMSCs (with well-characterized adipogenic, chondrogenic and osteogenic pathways). First, they incubated a monolayer of hMSCs with fluorescently-labelled analogues of thrombin; thereafter, they labeled the hMSCs with a plasma membrane-specific dye and imaged immediately to confirm the thrombin-plasma membrane binding. Using time-lapse confocal fluorescence microscopy, they showed the nucleation and fibrin growth from the cells thereafter. Welding with stem cells for next-generation surgical glues Advanced cell therapies are currently approaching clinical translation in response to an increasing demand for newly modified, cell-specific matrices (scaffolds) for biocompatible therapeutic performance. However, the rational design of matrices is extremely challenging since the cell phenotype and cell fate can be intertwined to a wide-range of scaffold-dependent factors; including cell adhesion, surrounding chemical composition, cell receptor stimulation, surface micro-/nano-morphology and mechanical stiffness. These factors immediately impact cells during in vitro tissue engineering, typically when cells are seeded to adhere on biocompatible and biodegradable scaffolds in the lab, where the scaffolds act as a surrogate extracellular matrix (ECM). Eventually, when the cells grow and differentiate, they can produce natural ECM of their own, to gradually replace the biomimetic scaffold material and form a structurally self-supporting biological entity. Deller et al. also completed cell growth assays to determine relative metabolic activities of labelled hMSCs-thrombin to show the modified cells were without observable cytotoxicity in varying concentrations of thrombin (1 µm to 25 mM). Using confocal microscopy again, the scientists showed the arrangement of fibrin structures emanating from the plasma membrane of the hMSC monolayer, in contrast to hMSCs without thrombin. The work protocol thereby generated a 3-D fibrin hydrogel construct with dense cellular aggregates surrounded by a 3-D fibrin matrix. The scientists also investigated the ability of the fibrin hydrogel system to sustain 3-D cultures for long-term growth; a requisite for tissue engineering, to show hMSC differentiation via adipogenic and osteogenic pathways. To verify the results, the scientists conducted extensive biomechanical tests on the cell types and tested for the upregulation of specific genes SOX9 and RUNX2 involved in chondrogenesis and osteogenesis respectively, using RT-PCR (reverse transcription polymerase chain reaction), to substantiate the fibrin hydrogel system sustained long-term hMSC proliferation in vitro.After confirming the membrane re-engineering approach for in lab tissue engineering applications, Deller et al. investigated the ability to produce thrombin coated cells in lab for their injection at a site of injury to initiate a healing response for tissue-engineering applications in vivo. For this, the scientists used an in vivo Zebrafish model system to perform preliminary cell transplant studies. Zebrafish is a model organism, established for fluorescently labelled live cell imaging and thrombolytic and hemostatic processes; suited for the present work. The scientists isolated, labelled and delivered fluorescently labelled primary Zebrafish fibroblasts, labelled with sc_thrombin[ox890] conjugate via microinjection to show cell survival after 3 days at a site of incisional injury. The synthesis and characterisation of the supercationic thrombin-polymer surfactant conjugate. a Schematic showing the electrostatic surface potential of native and supercationic thrombin (sc_thrombin) (PDB; 1UVS) at pH 7, highlighting the anionic (blue) and cationic (red) charged regions. Generation of the polymer surfactant corona (green halo) via electrostatic coupling of glycolic acid ethoxylate 4-nonylphenyl ether (ox890) to sc_thrombin gives [sc_thrombin][ox890]. b Zeta potential (ca. pH7; n = 3) of thrombin as a function of cationization times (0–120 min). Data reported as means ± standard deviation (s.d.). c Rate of fibrinogen solution (3.125 mg mL−1) gelation as measured by changes in turbidity (600 nm) catalysed by sc_thrombin subjected to various cationization times (0–120 min). Data shown as one-phase association curves of raw data. d MALDI-TOF MS spectra (m/z = 3) of native and sc_thrombin (60 min). Credit: Nature Communications, doi: 10.1038/s41467-019-09763-0 Schematic diagram showing in situ fibrin hydrogel formation from the membranes of bone-marrow derived human mesenchymal stem cells (hMSCs). Artificial membrane binding thrombin constructs comprising supercationic thrombin molecules (white) surrounded by a polymer surfactant corona (yellow) that associates with surface exposed cationic (red) residues spontaneously insert into bilayer regions of hMSC plasma membranes. In the presence of fibrinogen, the membrane-immobilised thrombin catalyses fibrin formation (blue fibres) within the interstitial spaces between the cells giving rise to a self-supporting hydrogel monolith. Credit: Nature Communications, doi: 10.1038/s41467-019-09763-0 More information: Robert C. Deller et al. Artificial cell membrane binding thrombin constructs drive in situ fibrin hydrogel formation, Nature Communications (2019). DOI: 10.1038/s41467-019-09763-0 D. E. Discher. Tissue Cells Feel and Respond to the Stiffness of Their Substrate, Science (2005). DOI: 10.1126/science.1116995 Tamer A.E. Ahmed et al. Fibrin: A Versatile Scaffold for Tissue Engineering Applications, Tissue Engineering Part B: Reviews (2008). DOI: 10.1089/ten.teb.2007.0435 © 2019 Science X Network In a recent study, Robert C. Deller and co-workers at the interdisciplinary departments of Cellular and Molecular Medicine, Engineering, Functional Nanomaterials and Pharmacology, and Neuroscience in the UK, bioengineered a self-contained cell matrix-forming system. In the experiments, they modified the plasma membrane of human mesenchymal stem cells (hMSCs) to integrate a new thrombin construct, which gave rise to spontaneous fibrin hydrogel nucleation and growth when supplemented with human plasma concentration levels of fibrinogen in cell culture media. The scientists bioengineered the cell membrane by synthesizing a membrane-binding supercationic thrombin-polymer surfactant complex. Thereafter, they observed cell differentiation in the resulting robust, stem cell-containing fibrin hydrogel constructs to form osteogenic and adipogenic cell lineages. The differentiated cells could eventually secrete fibrin to form self-supported bioengineered cellular monoliths that exhibited Young’s moduli as expected of the native extracellular matrix. The results are now published in Nature Communications. A range of natural biocompatible polymers have produced such transient hydrogel scaffolds for tissue engineering; including chitosan, gelatin and fibrin. Fibrin hydrogels are the most popular among them, since they can be produced readily at room temperature using proteolytic cleavage. Biological fibrin formation occurs in response to injury, culminating from a biochemical cascade of proteolytic cleavage, which converts prothrombin to thrombin and forms a fibrin-hydrogel clot. Fibrin hydrogels can therefore mediate cellular biomolecular functions and regulate the osteogenic and chondrogenic differentiation of human stem cells such as hMSCs. They can also be conveniently delivered using syringes, albeit with complications related to reduced cell viability.In the present work, Deller and co-workers first described a method to synthesize supercationic thrombin-polymer surfactant complexes that spontaneously bound to the plasma membrane of hMSCs to drive in situ fibrin hydrogel nucleation and growth. The resulting self-supporting hydrogel construct allowed high levels of metabolic activity as an artificial matrix for effective differentiation of stem cells to form adipogenic or osteogenic cell lineages. The scientists then showed the feasibility of the method of cell functionalization by injecting thrombin-labelled GFP-expressing fibroblasts (GFP: Green fluorescence protein) into a zebrafish (Danio rerio) skin wound model to demonstrate their in vivo biocompatibility for hemostatic applications. Explore further Journal information: Nature Communications In vivo zebrafish injury and [sc_thrombin][ox890] labelled GFP + fibroblast addition. Schematic representation of the in vivo adult zebrafish injury model. a Wildtype (non-transgenic) recipient zebrafish were anaesthetized and a 4 mm incisional injury made on the ventral upper thorax. A lateral view is shown. b Unlabelled or [sc_thrombin][ox890] labelled, FACS sorted GFP+ fibroblasts were injected at six sites around the edge of the incisional injury. At the desired time-point, fish were sacrificed and the tissue surrounding the incision was fixed, imaged and embedded for sectioning. A ventral view is shown. Ventral view of the area of tissue surrounding the incision at 3 dpi following transfer of c unlabelled or d [sc_thrombin][ox890] labelled GFP+ fibroblasts. Similar numbers of cells were retained at all wounds. The red line depicts the approximate position of the incisional injury which is fully re-epithelialized at this stage. Sections through the injury region at 3 dpi following transfer of e unlabelled or f [sc_thrombin][ox890] labelled GFP+ fibroblasts. No obvious differences were observed between wounds containing labelled or unlabelled cells. Arrows indicate the position of the incision. Credit: Nature Communications, doi: 10.1038/s41467-019-09763-0. Citation: Engineering artificial cell membranes to drive in situ fibrin hydrogel formation (2019, May 6) retrieved 18 August 2019 from https://phys.org/news/2019-05-artificial-cell-membranes-situ-fibrin.html Using macroscopic observations and histological assays, the scientists further revealed that there were no adverse effects between fish injected with engineered or native fibroblasts. However, Deller et al. expect to complete further investigations to understand the precise effects on the specific wound healing response using bioengineered cells in the future.In this way, Deller et al. synthesized and characterized a new membrane active thrombin construct and demonstrated its application to drive in situ fibrin formation in the plasma membranes of stem cells. The scientists showed that thrombin-based fibrin hydrogel constructs prepared using the new protocol supported high levels of cell growth and viability to eventually produce a self-supporting tissue engineered construct. The stem cells were also able to differentiate along the well-defined adipogenic and osteogenic pathways while demonstrating Young’s moduli similar to the native cells to indicate high levels of integration of the modifications. Deller et al. propose to optimize the protocols for further experiments in vitro prior to in vivo translation, to gain further insight to the enzymatic activity of cell membrane bound bioengineered proteins to develop biocompatible, hemostatic products. Evaluating rhodamine (rh) labelled rh_thrombin, rh_sc_thrombin and [rh_sc_thrombin][ox890] on hMSC plasma membrane affinity. Cells labelled with CellMask (green) and rhodamine labelled thrombin (magenta) visualized with confocal microscopy. Video shows the rh_sc_thrombin [ox890] labelled hMSCs supplemented with fibrin gel conjugating with fibrinogen (green) to highlight fibrin formation emanating from the bioengineered cell surface. Credit: Nature Communications, doi: 10.1038/s41467-019-09763-0 , Science This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.
Olive oil is the liquid goodness we reap from the olive. This tree crop is mostly found in the Mediterranean Basin and has a lot to offer to humankind. We use it in the kitchen, on our bodies, furniture and so many other places. Olive oil’s health benefits are commonly known but the interesting and gripping history behind it is rarely spoken about. Of the many theories behind the evolution of olive oil, two of the most interesting are: The Big Fat Greek Theory Also Read – Add new books to your shelfAthens is named for the Goddess Athena who brought the olive to the Greeks as a gift. Zeus had promised to give Attica to the god or goddess who made the most useful invention. Athena’s gift of the olive, useful for light, heat, food, medicine and perfume was looked greatly upon. Athena planted the original olive tree on a rocky hill that we know today as the Acropolis. The olive tree that grows there today is said to have come from the roots of the original tree. Homer, the Greek poet called it ‘liquid gold.’ Also Read – Over 2 hours screen time daily will make your kids impulsiveLight of JewsOlive oil lamp is the kind of lighting that was used in the Holy-land 2,000 years ago. It is the oil that was used in the Temple Menorah during both the first and the second Temple in Jerusalem. It was the oil that brought about the miracle of Hanukkah during the time of the Maccabees – over 150 years before Jesus was born and the oil that Jesus studied the Holy Scriptures by, in his childhood home in Nazareth.In Greece, they rubbed their bodies with this oil and it’s glow has illuminated it’s rich history, also the tree has been blessed with something close to immortality. They grow proud and strong no matter what harsh weather mother nature puts them through. This adds to it’s popularity , making it a household item. The benefits of olive oil are innumerable. From curing cancer cells to aiding hair growth to moisturizing the skin to removing the dirt from tiny nails. But an underrated concept of olive oil is its role in art. The main reason it is used for art is because Olive oil burns cleanly, without smoking. It doesn’t spread toxic hydro-carbons into the air. It is inexpensive and plentiful and a renewable lamp fuel. Instead of spending a fortune on decoration, using the bottle that contains the liquid gold is way more convenient. Olive Oil LampUsing pliers cut a 15 inch length of wire. Roll one end of the wire tightly around the nose of the pliers 4 to 6 times. Move to the other end of the wire and wrap a circle that will fit into the bottom of the jar that you’ve chosen for your lamp. Wrap this circle several times with the wire to make a firm foundation for your wick. Using tweezers, feed your wick through the circle made by the pliers, and pull through the top as in the photo. Place the wick in its holder in the jar. Fill with olive oil to within one inch of the top of the wick. Allow the wick to absorb olive oil to the top of the wick. This may take 10 minutes or more. Once the wick is saturated, light the wick with a match or candle and voila you have a olive oil lamp.Olive oil Painting Paint an empty wine bottle with non-toxic paint and fill with olive oil. Top with an oil pour spout that can be found at a gourmet cooking shop. Turn a wine bottle into an oil bottle Van Gogh on a wine bottleLemon-filled Olive Oil LanternsLoosely twist your wire around the wick leaving a small portion at the top in order to light on fire. Center your wick inside your container then bend the wire over the side to anchor it into place. You can buy special oil lantern wicks specifically to use with converting canning jars into lanterns. Then slice your lemons to your desired thickness and place them inside your container arranging them in a way to cover the sides without taking up the bulk of room inside the container. You want to fill it with as much oil as possible. After you’ve arranged your lemon slices the way you want, carefully fill the container with your oil. Not only are the lemons pretty but they also help scent the oil so it will give off a soft lemon fragrance the longer they soak in the oil.The real origin of the blessed olive is not certain but it is speculated to be from Syria or sub-Saharan Africa. For more than 6000 years, olive has developed alongside Mediterranean civilizations and is now commercially produced on more than 23 million acres in the Mediterranean basin. It is more just food or flavour for the people from the Basin. It is their identity and pride.Having been used for centuries, it is simply one of the least harmful fats in use no matter what our nutritional choices may be. It’s endorsement by all communities is the proof of it’s worth. Olive oil beauty products are a multibillion dollar business and its uses stretch far and wide – from human health to art – you name it, olive oil has a solution for it.
Thursday, September 1, 2016 MIAMI — Carnival Imagination is scheduled to go into dry dock Sept. 4 – 25 for an extensive multimillion dollar renovation aimed at upgrading the ship’s food and beverage concepts.The new spaces will be added over the course of the three weeks. Following its makeover Carnival Imagination will resume its year-round schedule of three- and four-day Baja cruises from Long Beach, California.The new additions will include:Guy’s Burger Joint, a free-of-charge poolside venue developed in partnership with Food Network personality Guy Fieri serving hand-crafted burgers and fresh-cut fries Travelweek Group RedFrog Rum Bar, a poolside watering hole with Caribbean rum-based frozen drinks and beers, including Carnival’s own private label draught brew, ThirstyFrog Red BlueIguana Tequila Bar, a festive outdoor bar offering a laid-back Mexican-themed atmosphere and tequila-based frozen drinks and beersCherry On Top with bins of bulk candy, fun gifts and novelties, custom apparel and more Posted by Alchemy Bar, a ‘cocktail pharmacy’-themed lounge where mixologists ‘prescribe’ concoctions from different elixirs and ingredients with creative monikers like Forty is the New Twenty and The Restorative Basil DropMore news: CIE Tours launches first-ever River Cruise Collection BlueIguana Cantina, a complimentary poolside Mexican eatery where guests can enjoy authentic, freshly made burritos and tacos on homemade tortillas, as well as an elaborate toppings and salsa bar Carnival Imagination heads to drydock for multimillion dollar reno Carnival Imagination already offers a WaterWorks aqua park with a 300-foot Twister slide, the Serenity adults-only retreat and the Seuss at Sea program in partnership with Dr. Seuss Enterprises.“Carnival Imagination provides a convenient, value-packed West Coast cruise option and with these new enhancements we’re making this fantastic ship even better,” said Christine Duffy, president of Carnival Cruise Line. << Previous PostNext Post >>
Share Canada Jetlines stages in-air protest against lack of airline competition << Previous PostNext Post >> Posted by Thursday, July 25, 2019 VANCOUVER — Canada Jetlines is standing its ground against what it says is decreased airline competition by staging a protest – in the sky.Using four planes and 18 skydivers, Jetlines’ airborne protest was staged to rally Canadians, investors and the Competition Bureau around the idea of increased competition and decreased airfares.In an official YouTube video of the publicity stunt, Javier Suarez, Jetlines’ CEO, says, “Flights in Canada are a rip-off because Canada is controlled by two airlines and the Competition Bureau is doing nothing to stop them.”He adds: “We are Jetlines, Canada’s first ultra-low-fare airline and we’re here today to send the duopoly a message in the place they believe they own: the sky.”Skydivers are then seen jumping out of a plane and holding up signs that read, “We want fair airfare now”, “Canada needs more airlines” and “Down with the duopoly.”The video then encourages viewers to go to Jetlines’ website at fightback.jetlines.ca to sign an online petition that the carrier says will be presented to the Canadian Competition Bureau.More news: Universal enhances popular Harry Potter vacation package with new perksIn an official release, Jetlines writes: “While several airlines have attempted to enter the Canadian market, the duopoly has pushed them out with short-term match-pricing at prices below their avoidable costs…The Competition Bureau is currently investigating WestJet and their subsidiary new airline for ‘predatory pricing’ to undercut new entrants. Under Canada’s competition laws, predatory pricing occurs when an incumbent with market power sets its prices below avoidable costs.”Suarez, who is urging consumers to show their support for more competition, says that Canada is the only developed country without a ULCC due to the fact that two high-cost airlines control approximately 85% of the domestic market.The low-cost carrier (LCC) versus ultra-low cost carrier (ULCC) designation may be a matter of degrees, but Swoop, in the market for more than a year now, certainly bills itself as a ULCC, as does Flair, while Air Canada Rouge is typically positioned as more of a LCC.“We know Canadians are fed up as there are between five and six million passenger trips per year by land over to the U.S. each year to fly on U.S. low-cost carriers, based out of northern U.S. airports, that in many cases only operate from those airports due to the robust Canadian passenger traffic,” he says.More news: GLP Worldwide introduces first-ever Wellness programsCanada Jetlines is scheduled to launch on Dec. 17 as ‘Canada’s first true Ultra-Low Cost Carrier airline’, with plans to operate flights across Canada to destinations in the U.S., Mexico and the Caribbean with an Airbus A320 fleet. Tags: Canada Jetlines Travelweek Group
Source = e-Travel Blackboard: N.J Passengers flying with All Nippon Airways, Cathay Pacific and Singapore Airlines can expect a raise in fares after Hong Kong’s Civil Aviation Department approved an increase in passenger fuel charges. The new charge will take effect from early next year and could reach a maximum of 129 HK dollars for domestic flights and 621 HK dollars on long haul flights, Trading Markets reported. Airlines believe the new surcharge could help recover losses from the fluctuations in fuel prices over the past year.
The EBU is conducting a series of subjective tests to gauge the impact of higher frame-rates for UHDTV services in Munich this week.The tests, conducted by the EBU’s Broadcast Technology Futures group, make up of the heads of research labs of the BBC, IRT, Rai and NHK, is conducting tests at he IRT’s facilities.The move follows subjective UHDTV resolution tests last February that revealed that higher resolutions would not be enough to create a clearly perceptible difference compared to HDTV.The latest round of tests coincides with work being carried out to investigate other parameters required to generate a more immersive experience with UHDTV. These include include higher dynamic range and extended colour space as well as frame rates.The tests are designed to address questions including whether the current UHDTV standard specified by the ITU, which includes only the 120Hz higher frame rate, needs to be revised to incorporate other rates that are multiples of 50Hz. The EBU said that early indications supported the idea that higher frame rages are more appreciated by observers than higher resolution.
Netflix will have more subscribers outside of the US by 2018, according to new research.IHS Markit noted Netflix’s subs base grew 30% between 2014 and 2015, and is forecasted to grow 21% this year, as the SVOD service reaches a “territorial expansion” peak.International subs will grow 38% this year, the research claimed, with more than 2.8 million of these coming from the 130 new territories Netflix expanded into in January when it went virtuallyglobal (notably excluding China).“At the moment, Netflix has 79.9 million total paying subscribers,” said Irina Kornilova, senior analyst at IHS Technology. “By 2018, international subscribers will over-take the number of subscribers in the US for the first time, and by 2020, Netflix will have 75 million international subscribers.”Furthermore, Netflix will break the 100 million subs mark by 2018.IHS claimed Netflix’s revenues would reach US$13 billion by 2020, with 53% coming from international markets. Domestic revenue will hit US$6.2 billion, with international taking around US$7 billion.Unsurprisingly, western Europe will be among the leading regions outside the US for Netflix by the end of this year, with more than six million subscribers in the UK. There will also be a combined 5.4 million subs from the Netherlands and the Nordics.Germany will reach 2.2 million paying subs by the end of 2020 despite strong competition from Amazon Prime Video, IHS predicted.Markets such as Poland and Turkey, which are expected to be soon ‘localised’ by Netflix, will drive further uptake in their surrounding regions, but the effect will not happen immediately, said IHS.“With the worldwide launch, Netflix has launched a platform upon which it can build and differentiate the service to fit specifics of every region in the future,” Kornilova said.“Netflix is starting this localisation process in Poland and Turkey this year. Subscribers in these countries can expect an addition of local languages to the user interface, subtitles and dubbing of content. This will help drive new subscriber numbers dramatically.”Localisation will continue through to 2018, with these new territories forecasted to grow paying membership 133% in 2017 and 62% the following year.Subscribers from countries Netflix debuted in this year will reach 19 million by 2020, IHS predicted.
The price pattern in silver was similar, except the sell-off after the price spike in New York on Thursday evening was much more intense—and the spike low didn’t occur until around 11:40 a.m. EDT in New York trading. The price bounced back quickly, but then traded quietly higher into the close. JPMorgan et al obviously wasted little time in getting silver back below the $21 spot price mark. The high and low tick were recorded as $21.315 and $20.78 in the September contract. Silver closed in New York yesterday at $20.89 spot, down 27 cents from Thursday’s close. Volume, net of July and August, was 36,000 contracts. When it does, it will be ugly The gold price showed signs of going parabolic in what had all the hallmarks of a ‘no ask’ market shortly after trading began at 6 p.m. EDT in New York on Thursday evening. But, as I mentioned in The Wrap yesterday, “da boyz” were at the ready—and within a couple of hours, the gold price was in full retreat. The low tick came about 9:15 a.m. in New York yesterday morning—and from there the price chopped quietly higher until shortly before 2 p.m. EDT. From there it traded basically flat into the 5:15 p.m. close. The high and low tick were recorded by the CME as $1,325.50 and $1,305.00 in the August contract. Gold closed in New York on Friday at $1,310.90 spot, down $7.30 from Thursday’s close. Volume, net of roll-overs, was around 98,000 contracts. The CME Daily Delivery Report showed that 25 gold and zero silver contracts were posted for delivery within the Comex-approved depositories on Tuesday. I was happy to see that after two days of withdrawals from GLD, there was an increase yesterday—as an authorized participant added 57,741 troy ounces. And as of 8:16 p.m. EDT yesterday evening, there were no reported changes in SLV. To tell you the truth, dear reader, I’m not expecting to see any deposits into SLV for a considerable period of time, as the authorized participants are still attempting to cover their short positions in lieu of metal they never deposited during the June rally. The U.S. Mint had a tiny sales report yesterday as 1,000 troy ounces of gold eagles were sold—and 30,000 silver eagles. Month to date the mint has sold 24,500 troy ounces of gold eagles—4,000 one-ounce 24K gold buffaloes—and a pitiful 1,025,000 silver eagles. Ted Butler nailed this a month ago, as it’s obvious to anyone who wishes to objectively examine the U.S. Mint data, that silver eagles sales have crashed by at least two thirds in July, as the ‘big buyer’ that has been sucking up silver eagles [and Canada’s silver maple leaf] for the last several years, has obviously stepped away from the table for the moment. Whether this is going to turn into a permanent withdrawal remains to be seen—and because the Royal Canadian Mint only provides quarterly sales reports for their bullion products—we won’t know what’s going on there for about another three months. But the crashing silver eagles sales don’t bode well for silver maple leaf sales going forward, either. It’s certainly my suspicion that it’s the same buyer at the trough in both. Over at the Comex-approved depositories on Thursday, there was a decent amount of gold received—103,561 troy ounces to be exact. Virtually all of it went into the Manfra, Tordella & Brookes, Inc. depository. Nothing was reported shipped out. The link to that activity is here. It was another big day in silver again, as nothing was reported received, but 999,492 troy ounces were shipped out the door. All the activity was at the CNT Depository—and HSBC USA. The link to that action is here. Since the 20th of the July falls on a weekend, the always punctual and predictable Central Bank of the Russia Federation updated their website with June’s data on Friday. Included in that update was the amount of gold bullion they purchased for their reserves that month. It turned out to be a chunky 500,000 troy ounces. It was more or less the same chart pattern in the silver stocks, but because “da boyz” were more aggressive with silver to the downside, the rally off their 1:30 p.m. lows was only able to get Nick Laird’s Intraday Silver Sentiment Index back up to a loss of 0.70%. The gold stocks gapped down a bit less than 2 percent at the open—and then chopped sideways until the 1:30 p.m. Comex close. A rally commenced at that point which lasted right into the close, as the HUI cut its losses on the day to only 0.30%. Platinum spiked up as well, but also got sold down until about noon in Zurich. The subsequent rally ended/got capped shortly before 9 a.m. in New York—and from there it got sold down to its low of that day, around 1 p.m. EDT. From there it rallied a few dollars into the close. Platinum closed down 13 bucks on the day. The palladium price chart was a mini version of the platinum price chart. Palladium closed down only 5 bucks from Thursday’s close. In the last three months, the central bank has purchased 1,500,000 troy ounces of gold, which is pretty close to 100 percent of their own production. If you look at Nick Laird’s excellent chart above, you’ll note that Russia has stepped up its gold purchases in the last three months. One wonders if that has anything to do with the Crimea/Ukraine situation? Now if they could be convinced to buy all their silver production as well, then the fox would certainly be amongst the pigeons, as Russia’s 1,700 tonne yearly production represents a bit over 6.5 percent of yearly world silver production, which is a material amount. Well, the Commitment of Traders Report for positions held at the close of Comex trading on Tuesday, July 15 was certainly not what I had hoped for, at least in silver. The Commercial net short position in silver increased again, this time by 678 contracts, or 3.4 million ounces. The Commercial net short position now sits at 293.5 million troy ounces. The Big 4 trader’s short holdings [read JPMorgan] increased by around 1,200 contracts—and Ted Butler pegs JPMorgan’s short-side corner in the Comex silver market at about 19,000 contracts, or 95 million troy ounces. The ‘5 through 8’ largest short holders covered about 3,000 contracts of their short position during the reporting week. In gold, the Commercial net short position actually improved by 9,097 contracts, or 909,700 troy ounces. The Commercial net short position in gold has obviously declined by that amount—and is down to 15.69 million troy ounces, which is still a horrendously large number. The 8 largest short holders added 1,000 contracts to their short positions—and Ted says that JPMorgan sold another 3,000 contracts during the reporting week—and their long-side corner in the Comex gold market continues to shrink, and is now down to 2.2 million troy ounces, or 22,000 Comex contracts. Ted said—and I agree—that probably not all of the decline on Monday and Tuesday was reported to the CFTC in a timely manner, so hopefully there’s some spill-over into next Friday’s Report. If that doesn’t prove to be the case however, it’s a given that next Friday’s COT Report will be even uglier than even I imagined it might be, because in my comments in The Wrap yesterday, I stated that JPMorgan et al threw everything they had at that spike in gold and silver prices in New York Thursday morning. That data alone should be enough to curl your hair. Of course we have two more reporting days between now and the Tuesday cut-off—and anything can happen between now and then—but as it stands at the moment, the next COT will be pretty horrific, because almost the entire technical fund short positions in both metals are still in place, plus there will be more to add. This does not bode well for gold and silver prices somewhere down the road. Here’s Nick Laird’s “Days of World Production to Cover Comex Short Positions“—and it looks just as grotesque as it always does in all four precious metals. It’s also obvious that the situation in the precious metal market—and particularly in silver—is getting stranger by the day. 1] There’s no physical silver available to deposit in SLV, so the authorized participants have had to short the shares in lieu of depositing real metal. 2] The frantic in/out movement in silver within the Comex-approved warehouse system is approaching the absurd. According to Ted Butler’s calculations from the Comex warehouse reports, the extrapolated turnover year-to-date is somewhere between 200 and 300 million troy ounces per year at the moment. 3] The big buyer of silver eagles [and probably silver maple leafs as well] has stepped away from the table. Silver eagles sales have imploded as a result—and we’ll find out in October whether the same applies to silver maple leafs. 4] The silver charts show a neutral RSI, but the Commercial net short position is sky high—and back where it was about four years ago—and the technical funds net long positions are almost at a record high. 5] With all of this going on, silver is sitting under $21 the ounce—and below the cost of production of most primary silver producers. One can scarcely imagine what the price will be when JPMorgan et al get through harvesting this near-record technical fund long position for fun, profit and price management purposes. How all this is going to resolve itself—and over what time period—is unknown, but when it does, it will be ugly. How did it come to this? After almost twelve hours of writing this column, I’m done for the day—and the week. Enjoy what’s left of your weekend—and I’ll see you on Tuesday. I have a decent number of stories for you today—and I hope you can find the time in what’s left of your weekend to read the ones you like. “The herd instinct among forecasters makes sheep look like independent thinkers.” – Edgar R. Fiedler, author of The Three Rs of Economic Forecasting—Irrational, Irrelevant and Irreverent Today’s pop ‘blast from the past’ is by a Canadian rock group that needs no introduction, as their name is known world-wide. This hit dates from the early 1970s—and has an unusual story behind it. The link is here. Today’s classical ‘blast from the past’ is an old chestnut from Peter I. Tchaikovsky. It’s the Polonaise from his opera Eugene Onegin. I’d be the most surprised person in the world if you haven’t heard this piece in one form or another during your lifetime. The link is here. Except for the brief price spikes shortly after the precious metal market opened early in Far East trading on their Friday, it was a nothing sort of day all around. But it should be obvious to all but the willfully blind that “da boyz” were involved in gold and silver yesterday when they had to be. Here are the 6-month gold and silver charts updated with Friday’s data. Sponsor Advertisement The dollar index closed at 80.53 late on Thursday afternoon in New York and, like Thursday, didn’t do much during its respective trading session. It chopped sideways in a 2 basis point range until around 9:40 a.m. EDT, when a spike took it up to 80.68—but by 2 p.m. it was back to unchanged on the day—and that’s where it closed, at 80.53. That’s the third day in a row that the dollar index has closed at that value. Here’s the 3-day chart so you can see it for yourself. Avrupa and Antofagasta intersect copper-rich VMS in Pyrite Belt, Portugal • First Greenfields discovery of massive sulfide mineralization in 20 years in the Iberian Pyrite Belt • 10.85 meters of massive and semi-massive/stockwork sulfide mineralization grading 1.81% Cu, 2.57% Pb, 4.38% Zn, 0.13% Sn, and 75.27 ppm Ag • Including 7.95 meters @ 2.21% Cu, 3.05% Pb, 4.82% Zn, 0.15% Sn, 89.8 ppm Ag • Followed by 2.90 meters @ 0.71% Cu, 1.27% Pb, 3.17% Zn, 0.092% Sn, 35.4 ppm Ag • Avrupa and Antofagasta sign an amended Joint Venture Agreement Please visit our website to learn more about the company and current exploration program.
Discount-49%-36%-34% Looking at this table, emerging market stocks might seem like a no-brainer. But let’s be honest…• You should have to pay more for U.S. stocks…After all, the United States is still the most powerful and stable economy on the planet. And investors pay premiums for safety.Emerging markets, on the other hand, are far less stable.China, the world’s biggest emerging market, is a communist country. Brazil, another huge emerging market, has had five currency crises in the last eight decades.In short, emerging market stocks come with heavy baggage. Because of these risks, many investors want to “be paid extra” for owning emerging markets. They want better returns or higher yields.With this in mind, you have to ask yourself: Are emerging market stocks worth the risk?• Emerging market economies are growing rapidly…According to the International Monetary Fund (IMF), emerging markets grew 4.1% last year. For perspective, the U.S. economy grew 1.6%.This year, the IMF expects emerging markets to grow 4.5%. It expects the U.S. economy to grow 2.3%.For 2018, the IMF projects that emerging markets will grow 4.8%, compared to 2% for the U.S. economy.In other words, emerging market stocks are cheap and offer more growth potential. This is what every investor looks for.But this has been true about emerging markets for years. And yet, they’ve basically done nothing while U.S. stocks have soared to record highs.What would make this time any different?• Commodity prices have taken off…Palladium is up 14% this year. Silver’s up 12%. Copper has gained 9%.Keep in mind, commodities have been falling for the better part of the last six years. The Bloomberg Commodity Index (BCOM), which tracks 22 commodities, declined 58% between April 2011 and last January. Since then, it’s up 21%.This has given emerging market stocks a huge boost.You see, countries like Brazil, Russia, Venezuela, and Saudi Arabia export far more commodities than they import.When commodities rise, these exporters make more money. Their economies grow faster. Their stock markets climb higher.Higher commodity prices could be the catalyst that emerging market stocks have been waiting for. But that doesn’t mean you should blindly invest in them.Tomorrow, we’ll tell you what we don’t like about emerging market stocks.At the end of that issue, you’ll know whether emerging market stocks deserve a spot in your portfolio.Chart of the Day: How “Broad” Is Your Exposure?China is driving the rally in emerging market stocks.Earlier we told you that China is the biggest emerging market economy. It’s also by far the largest holding in EEM. It makes up 26% of the index. It has more impact on the fund than India, Brazil, Russia, and Mexico combined.The chart below shows the performance of the iShares China Large-Cap ETF (FXI), which tracks large Chinese stocks, since last April. If it looks familiar, it’s because FXI has moved almost in lockstep with EEM over the same period.This is important to understand.You see, a lot of investors buy emerging market ETFs thinking they’re getting broad emerging market exposure. But many of these funds are heavily concentrated in big countries like China.In short, if you own EEM, you’d better be bullish on China.Regards,Justin SpittlerDelray Beach, FloridaFebruary 16, 2017We want to hear from you.If you have a question or comment, please send it to firstname.lastname@example.org. We read every email that comes in, and we’ll publish comments, questions, and answers that we think other readers will find useful. EEM1.3411.871.31 S&P 5002.6518.681.97 Emerging market stocks have been “dead money” for almost a decade.Emerging markets are countries that are on their way to becoming “developed” like the United States or Germany. Brazil, Russia, India, and China—the so-called “BRIC” countries—are the biggest emerging markets.More than 80% of the world’s population lives in these countries. Since 2008, these economies have accounted for 80% of the growth in global economic trade and output.You would think this would have made them great investments. But emerging markets have actually been a horrible investment lately…Take a look at the chart below.It shows how the iShares MSCI Emerging Markets ETF (EEM), which tracks more than 800 emerging market stocks, performed from 2007 through 2015. You can see that it went nowhere.You would have actually lost about 0.15% of your money if you held EEM over this period, and that includes dividends.• Because of this, many investors have given up on emerging market stocks…But that could soon change.Last year, EEM gained 8.6%. It was its first annual gain since 2012.This year, it’s already up 10%. That’s more than double the S&P 500’s 4% gain.More importantly, it looks like EEM just “broke out.” Below, you can see it recently bucked a downtrend it’d been stuck in since early September.• This is good news for emerging market stocks…As we often point out, stocks usually keep rising after a breakout like this.And that’s exactly what EEM’s done. It’s rallied about 6% since piercing its downtrend.It’s now at the highest level since July 2015.Of course, you probably want to know if emerging market stocks will keep rising.Over the next couple days, we’re going to try to answer that question.We’ll dive deep into the fundamentals of emerging market stocks. We’ll look at the good and the bad. By the end, you’ll know if emerging market stocks are right for you.Let’s start with what we like about them…• Emerging market stocks are much cheaper than U.S. stocks…You can see this in the table below.This table compares EEM with the SPDR S&P 500 ETF (SPY), which tracks companies in the S&P 500.EEM’s price-to-book (P/B) ratio is 49% lower than SPY’s P/B ratio. Its forward price-to-earnings (forward P/E) ratio is 36% lower. It’s also 34% cheaper according to the price-to-sales (P/S) ratio. Price/Book(P/B) Forward Price/Earnings(Forward P/E)Price/Sales(P/S)
An E. coli outbreak that sickened people in 36 states and triggered warnings not to eat romaine lettuce this spring has been traced to water in a canal in the Yuma, Ariz., region – and the outbreak is now officially over, federal officials say.”Suspect product is no longer being harvested or distributed from this area and is no longer available in stores or restaurants, due to its 21-day shelf life,” the Food and Drug Administration says.Five people have died because of the outbreak and 96 were sent to hospitals, the FDA says in its latest update. Overall, the agency says, 210 people were made ill by the E. coli outbreak.Alarm over the outbreak was relaxed somewhat in late May, after regulators confirmed that the harvesting season for romaine in Yuma had passed, and that the main U.S. source for romaine had shifted to California’s Salinas Valley. The first cases in the outbreak were reported on March 13; one month later, the Centers for Disease Control and Prevention said it had traced the E. coli to lettuce that was grown in the area around Yuma.Citing the CDC’s analysis of water samples that were taken from a canal in the Yuma region, the FDA says the investigation found E. coli in the water “with the same genetic finger print as the outbreak strain.”Investigators are now working to learn how the E. coli got into the water, and how the water, in turn, contaminated the romaine lettuce from several farms.As he announced the news of the breakthrough in tracing the cause of the illnesses, FDA Commissioner Scott Gottlieb also addressed the notion that U.S. consumers are seeing more foodborne outbreaks than they have in the past.”The answer to that question is that we don’t believe we are seeing more outbreaks,” Gottlieb said. “In fact, we believe food is safer than perhaps ever before and today we’re better at finding outbreaks when they occur.” Copyright 2018 NPR. To see more, visit http://www.npr.org/.
Learn from renowned serial entrepreneur David Meltzer how to find your frequency in order to stand out from your competitors and build a brand that is authentic, lasting and impactful. Enroll Now for $5 The rules for dealing with violent and disturbing images often require moderators to ask whether they are ‘newsworthy’ or ‘raise awareness.’ May 22, 2017 Image credit: JaysonPhotography / Shutterstock.com Add to Queue Entrepreneur Staff Nina Zipkin 3 min read Fireside Chat | July 25: Three Surprising Ways to Build Your Brand Staff Writer. Covers leadership, media, technology and culture. Next Article There is no doubt that it takes a huge effort to moderate all the content that gets uploaded to Facebook. But over the past few months, the social giant has shown signs of strain.Back in August, shortly after the company fired a team of human editors overseeing the Trending section of the site in favor of an algorithm, a false news story found its way to the top of the queue.In February, CEO Mark Zuckerberg published a wide-ranging open letter on his Facebook page about the direction he hopes to take the company, touching on the need for more vigilance in the face of “fake news” and also a stronger infrastructure to handle the raft of content that is posted by users on a daily basis.Related: After Murder, Facebook to Hire 3,000 People to Review Videos“There are billions of posts, comments and messages across our services each day, and since it’s impossible to review all of them, we review content once it is reported to us,” Zuckerberg wrote. “There have been terribly tragic events — like suicides, some live streamed — that perhaps could have been prevented if someone had realized what was happening and reported them sooner. There are cases of bullying and harassment every day, that our team must be alerted to before we can help out. These stories show we must find a way to do more.”This spring, after a murder in Cleveland was livestreamed on the platform, Zuckerberg announced that over the course of the year, 3,000 people would be hired to better tackle and improve that review process.But now, an investigation conducted by the Guardian has identified some of the standards that Facebook operates from when it comes to moderating content, and they are perhaps more confusing than you might expect.Related: Facebook Wants to Help You Spot Bogus News StoriesWith regard to the videos of violent deaths or suicides, they are designated as disturbing content, but the reasoning Facebook has for not necessarily taking them down is because they can build awareness about mental illness, according to The Guardian’s findings.Specifically in cases of suicide, documents that The Guardian has been privy to explain that the current company dictate is “to remove them once there’s no longer an opportunity to help the person. We also need to consider newsworthiness, and there may be particular moments or public events that are part of a broader public conversation that warrant leaving up.”When it comes to violent language, a call to action to harm the president would be taken down because he is a head of state, but directions about how to snap a woman’s neck would be allowed to remain on the site because it is not “regarded as credible threats.”Related: Facebook Pledges to ‘Do Better’ After Posting of Murder VideoFor instances of animal abuse and graphic violence, those images and videos are also designated as disturbing, but are allowed if they are being used to educate and raise awareness, but they are not if there is an element of “sadism and celebration.” For images or photos pertaining to child abuse, that rule is also applied.According to the Guardian, moderators often have seconds to make a determination about how to characterize or whether to remove the content.It’s clear that Zuckerberg and his team have a daunting task in front of them, so Facebook’s rules will need to constantly evolve to meet the challenge. –shares Facebook Facebook’s Content Moderation Rules Are Both Careful and Shocking