Officials complete tour of damage at HJ Robinson school resumes Monday Feb

first_img Education Minister tours schools this week Education Minister touts new ICT platform for Public Schools Ministry of Education introduces pilot program Related Items:akierra missick, HJ robinson high school, school resume Facebook Twitter Google+LinkedInPinterestWhatsApp Recommended for you Facebook Twitter Google+LinkedInPinterestWhatsAppProvidenciales, 04 Feb 2016 – A report from the Public Works Department is expected by the end of the week after inspection on Wednesday of the damage at the HJ Robinson High school; a fire gutted the top floor of the Administration Block. The assessment led by the Deputy Premier, Hon Akierra Missick was emotional and the Education Minister said her heart goes out to all directly affected by the January 29th fire. Along with furniture and fixtures, computers and the actual structure being damaged – years and years of students’ records were lost in the blaze which erupted around 3am. HJ did not reopen this morning; instead the advice is for school to resume for the 350 students on Monday February 8, 2016. The structural report to come from Public Works will say what is needed for the design and costing; then the job will go to tender. Minister Missick reiterated that government is committed to begin reconstruction as soon as possible; the DP also cautioned, however, that it took some time to amass these structural and educational assets of the Helena Jones Robinson High school; one of the country’s oldest institutions.last_img read more

Police investigating suspicious death of Sharp Grossmont Hospital patient

first_img KUSI Newsroom KUSI Newsroom, LA MESA (KUSI) — An investigation was underway Friday into the suspicious death of a man at Sharp Grossmont Hospital in La Mesa, police said.The 58-year-old patient died a little before 2 p.m. Thursday at the hospital on Grossmont Center Drive, La Mesa police Lt. Vince Brown said.Police did not disclose a reason the death was considered suspicious but said no hospital employees are considered suspects.“Hospital staff reported the incident to La Mesa Police and officers responded to the hospital … at approximately 1:58 p.m.,” Brown said.“Detectives were later called to the scene. No hospital employees are considered subjects in this investigation.”Police did not release any other details, including the name of the person who died, but asked anyone with information about the incident to call La Mesa police at (619) 667-1400, San Diego County Crime Stoppers at (888) 580- 8477 or contact the agency online at Tipsters may remain anonymous and could be eligible for a reward of up to $1,000. Posted: December 29, 2017 Updated: 4:07 PMcenter_img December 29, 2017 Police investigating suspicious death of Sharp Grossmont Hospital patient Categories: Local San Diego News Tags: La Mesa Police, Sharp Grossmont Hospital FacebookTwitterlast_img read more

Paper Makers Make More Cuts

first_img“This was an attractive opportunity, and we intend to use the proceeds received at closing to pay down debt,” IP’s chairman and CEO John Faraci said in a statement. The down economy and reduced demand have taken a toll on paper makers. One producer, Sappi Fine Paper North America, announced that it will indefinitely suspend operations at its facility in Muskegon, Michigan, resulting in the furlough of approximately 190 salaried and hourly employees. The suspension is set to begin April 1.The company said the suspension was necessary in light of “significantly lower global demand for coated fine paper products.” As part of a separate cost-cutting initiative, Sappi also said it is eliminating an additional 70 positions company-wide.UPM is suspending production at a pair of its mills in Finland in April. The suspension is said to result in a 880,000-ton per-year reduction of coated and uncoated specialty paper as well as uncoated mechanical magazine paper.Meanwhile, Memphis, Tennessee-based International Paper said it is divesting the equivalent of 143,000 acres of properties located in southwestern U.S. The company is selling 114,000 acres to the American Timberlands Fund for $220 million in cash and donated the remaining acres (worth approximately $55 million) in exchange for a 20 percent investment in the fund.last_img read more

Hard Rock Cafes India franchisee to raise fund expand outlets

first_imgJSM Corp Ltd, which has Hard Rock Café franchisee in India, is planning to raise Rs. 200 crore from private equity investors. The funds raised would be used to expand the number of outlets and bring in more international brands.The company, which also runs popular restaurant chains California Pizza Kitchen and Shiro in India, is considering fund raising by older investors selling their shares. Lodha Capital Markets is acting as the company’s adviser. As part of the company’s expansion plans, JSM would launch popular Chinese food chain Panda Express in India by August 2016, the Mint reported.”About Rs. 150 crore will be raised primarily from new investors, while Rs. 50 crore will be a secondary transaction where the legacy investors will be brought over,” Jay Sing, co-founder and executive director of JSM, was quoted as saying by the publication.JSM Corp was founded by Jay Singh and Sanjay Mahtani in 2004. Some of the other brands of the company are The Big Kahuna, Ginger Tiger, dessert chain Pinkberry, street-food chain Plus91 and Asilo, which is a rooftop bar in Mumbai, according to the company’s website. Singh and Mahtani together hold 55 percent stake in JSM Corp, while the rest is owned by other investors.Azim Premji’s private equity arm, PremjiInvest, acquired 22 percent stake in JSM for about Rs. 150 crore four years ago. The same PE firm may put in additional funds, Singh was quoted as saying by the Mint.A report by consulting firm Grant Thornton India and the Federation of Indian Chambers and Commerce Industry (FICCI) said the Indian food and beverage industry will expand at a pace of 24 percent every year to reach Rs. 3.8 trillion in sales by March 2017.[1 lakh = 100,000 | 1 crore = 10 million | 100 crore = 1 billion]last_img read more

Engineering artificial cell membranes to drive in situ fibrin hydrogel formation

first_imgRe-engineering the cell membrane for improved biofunction is an emerging, powerful tool in cell biology to develop next-generation cell therapies. The process can allow users to supplement cells with added therapeutic functionalities. Additional functionalities can include cell homing, surface adhesion or resistance to hypoxia for enhanced cellular capabilities. However, the number of such examples on re-engineered plasma-membranes to activate membrane-bound enzymes that promote the assembly of extracellular matrix (ECM) proteins to promote cell functionalities are limited. 3D projection of fibrin gel containing fibrinogen stained with Alexa 594 (red) fibrinogen and hMSCs incorporating sc_thrombin [ox890] stained with Hoeschst 33342, imaged after 60 min of cell associated fibrin formation in cell culture. Credit: Nature Communications, doi: 10.1038/s41467-019-09763-0. They synthesized the artificial membrane binding thrombin complex using a two-step process to generate an active supercationic thrombin construct (sc_thrombin). Deller et al. also generated a polymer surfactant corona or green halo using electrostatic coupling of glycolic acid ethoxylate 4-nonylphenyl ether (ox890) to sc_thrombin to engineer a third variant sc_thrombin [ox890]. The scientists controlled the reaction conditions (pH, temperature and chemical composition) carefully and monitored the reaction progress using zeta potentiometry across a period of two hours. They observed the activity of thrombin by monitoring/characterizing the increasing turbidity of the fibrinogen solution. They then obtained MALDI-TOF spectra (matrix-assisted laser desorption ionization time of flight mass spectroscopy) of the native and modified thrombin to show full cationization of the construct. When Deller et al. conducted compression testing of the resultant self-supporting structures, the Young’s moduli were similar to soft fibrin hydrogels, indicating consistency. To investigate thrombin adhesion to cell membranes, the scientists chose a monolayer of bone marrow derived hMSCs (with well-characterized adipogenic, chondrogenic and osteogenic pathways). First, they incubated a monolayer of hMSCs with fluorescently-labelled analogues of thrombin; thereafter, they labeled the hMSCs with a plasma membrane-specific dye and imaged immediately to confirm the thrombin-plasma membrane binding. Using time-lapse confocal fluorescence microscopy, they showed the nucleation and fibrin growth from the cells thereafter. Welding with stem cells for next-generation surgical glues Advanced cell therapies are currently approaching clinical translation in response to an increasing demand for newly modified, cell-specific matrices (scaffolds) for biocompatible therapeutic performance. However, the rational design of matrices is extremely challenging since the cell phenotype and cell fate can be intertwined to a wide-range of scaffold-dependent factors; including cell adhesion, surrounding chemical composition, cell receptor stimulation, surface micro-/nano-morphology and mechanical stiffness. These factors immediately impact cells during in vitro tissue engineering, typically when cells are seeded to adhere on biocompatible and biodegradable scaffolds in the lab, where the scaffolds act as a surrogate extracellular matrix (ECM). Eventually, when the cells grow and differentiate, they can produce natural ECM of their own, to gradually replace the biomimetic scaffold material and form a structurally self-supporting biological entity. Deller et al. also completed cell growth assays to determine relative metabolic activities of labelled hMSCs-thrombin to show the modified cells were without observable cytotoxicity in varying concentrations of thrombin (1 µm to 25 mM). Using confocal microscopy again, the scientists showed the arrangement of fibrin structures emanating from the plasma membrane of the hMSC monolayer, in contrast to hMSCs without thrombin. The work protocol thereby generated a 3-D fibrin hydrogel construct with dense cellular aggregates surrounded by a 3-D fibrin matrix. The scientists also investigated the ability of the fibrin hydrogel system to sustain 3-D cultures for long-term growth; a requisite for tissue engineering, to show hMSC differentiation via adipogenic and osteogenic pathways. To verify the results, the scientists conducted extensive biomechanical tests on the cell types and tested for the upregulation of specific genes SOX9 and RUNX2 involved in chondrogenesis and osteogenesis respectively, using RT-PCR (reverse transcription polymerase chain reaction), to substantiate the fibrin hydrogel system sustained long-term hMSC proliferation in vitro.After confirming the membrane re-engineering approach for in lab tissue engineering applications, Deller et al. investigated the ability to produce thrombin coated cells in lab for their injection at a site of injury to initiate a healing response for tissue-engineering applications in vivo. For this, the scientists used an in vivo Zebrafish model system to perform preliminary cell transplant studies. Zebrafish is a model organism, established for fluorescently labelled live cell imaging and thrombolytic and hemostatic processes; suited for the present work. The scientists isolated, labelled and delivered fluorescently labelled primary Zebrafish fibroblasts, labelled with sc_thrombin[ox890] conjugate via microinjection to show cell survival after 3 days at a site of incisional injury. The synthesis and characterisation of the supercationic thrombin-polymer surfactant conjugate. a Schematic showing the electrostatic surface potential of native and supercationic thrombin (sc_thrombin) (PDB; 1UVS) at pH 7, highlighting the anionic (blue) and cationic (red) charged regions. Generation of the polymer surfactant corona (green halo) via electrostatic coupling of glycolic acid ethoxylate 4-nonylphenyl ether (ox890) to sc_thrombin gives [sc_thrombin][ox890]. b Zeta potential (ca. pH7; n = 3) of thrombin as a function of cationization times (0–120 min). Data reported as means ± standard deviation (s.d.). c Rate of fibrinogen solution (3.125 mg mL−1) gelation as measured by changes in turbidity (600 nm) catalysed by sc_thrombin subjected to various cationization times (0–120 min). Data shown as one-phase association curves of raw data. d MALDI-TOF MS spectra (m/z = 3) of native and sc_thrombin (60 min). Credit: Nature Communications, doi: 10.1038/s41467-019-09763-0 Schematic diagram showing in situ fibrin hydrogel formation from the membranes of bone-marrow derived human mesenchymal stem cells (hMSCs). Artificial membrane binding thrombin constructs comprising supercationic thrombin molecules (white) surrounded by a polymer surfactant corona (yellow) that associates with surface exposed cationic (red) residues spontaneously insert into bilayer regions of hMSC plasma membranes. In the presence of fibrinogen, the membrane-immobilised thrombin catalyses fibrin formation (blue fibres) within the interstitial spaces between the cells giving rise to a self-supporting hydrogel monolith. Credit: Nature Communications, doi: 10.1038/s41467-019-09763-0 More information: Robert C. Deller et al. Artificial cell membrane binding thrombin constructs drive in situ fibrin hydrogel formation, Nature Communications (2019). DOI: 10.1038/s41467-019-09763-0 D. E. Discher. Tissue Cells Feel and Respond to the Stiffness of Their Substrate, Science (2005). DOI: 10.1126/science.1116995 Tamer A.E. Ahmed et al. Fibrin: A Versatile Scaffold for Tissue Engineering Applications, Tissue Engineering Part B: Reviews (2008). DOI: 10.1089/ten.teb.2007.0435 © 2019 Science X Network In a recent study, Robert C. Deller and co-workers at the interdisciplinary departments of Cellular and Molecular Medicine, Engineering, Functional Nanomaterials and Pharmacology, and Neuroscience in the UK, bioengineered a self-contained cell matrix-forming system. In the experiments, they modified the plasma membrane of human mesenchymal stem cells (hMSCs) to integrate a new thrombin construct, which gave rise to spontaneous fibrin hydrogel nucleation and growth when supplemented with human plasma concentration levels of fibrinogen in cell culture media. The scientists bioengineered the cell membrane by synthesizing a membrane-binding supercationic thrombin-polymer surfactant complex. Thereafter, they observed cell differentiation in the resulting robust, stem cell-containing fibrin hydrogel constructs to form osteogenic and adipogenic cell lineages. The differentiated cells could eventually secrete fibrin to form self-supported bioengineered cellular monoliths that exhibited Young’s moduli as expected of the native extracellular matrix. The results are now published in Nature Communications. center_img A range of natural biocompatible polymers have produced such transient hydrogel scaffolds for tissue engineering; including chitosan, gelatin and fibrin. Fibrin hydrogels are the most popular among them, since they can be produced readily at room temperature using proteolytic cleavage. Biological fibrin formation occurs in response to injury, culminating from a biochemical cascade of proteolytic cleavage, which converts prothrombin to thrombin and forms a fibrin-hydrogel clot. Fibrin hydrogels can therefore mediate cellular biomolecular functions and regulate the osteogenic and chondrogenic differentiation of human stem cells such as hMSCs. They can also be conveniently delivered using syringes, albeit with complications related to reduced cell viability.In the present work, Deller and co-workers first described a method to synthesize supercationic thrombin-polymer surfactant complexes that spontaneously bound to the plasma membrane of hMSCs to drive in situ fibrin hydrogel nucleation and growth. The resulting self-supporting hydrogel construct allowed high levels of metabolic activity as an artificial matrix for effective differentiation of stem cells to form adipogenic or osteogenic cell lineages. The scientists then showed the feasibility of the method of cell functionalization by injecting thrombin-labelled GFP-expressing fibroblasts (GFP: Green fluorescence protein) into a zebrafish (Danio rerio) skin wound model to demonstrate their in vivo biocompatibility for hemostatic applications. Explore further Journal information: Nature Communications In vivo zebrafish injury and [sc_thrombin][ox890] labelled GFP + fibroblast addition. Schematic representation of the in vivo adult zebrafish injury model. a Wildtype (non-transgenic) recipient zebrafish were anaesthetized and a 4 mm incisional injury made on the ventral upper thorax. A lateral view is shown. b Unlabelled or [sc_thrombin][ox890] labelled, FACS sorted GFP+ fibroblasts were injected at six sites around the edge of the incisional injury. At the desired time-point, fish were sacrificed and the tissue surrounding the incision was fixed, imaged and embedded for sectioning. A ventral view is shown. Ventral view of the area of tissue surrounding the incision at 3 dpi following transfer of c unlabelled or d [sc_thrombin][ox890] labelled GFP+ fibroblasts. Similar numbers of cells were retained at all wounds. The red line depicts the approximate position of the incisional injury which is fully re-epithelialized at this stage. Sections through the injury region at 3 dpi following transfer of e unlabelled or f [sc_thrombin][ox890] labelled GFP+ fibroblasts. No obvious differences were observed between wounds containing labelled or unlabelled cells. Arrows indicate the position of the incision. Credit: Nature Communications, doi: 10.1038/s41467-019-09763-0. Citation: Engineering artificial cell membranes to drive in situ fibrin hydrogel formation (2019, May 6) retrieved 18 August 2019 from Using macroscopic observations and histological assays, the scientists further revealed that there were no adverse effects between fish injected with engineered or native fibroblasts. However, Deller et al. expect to complete further investigations to understand the precise effects on the specific wound healing response using bioengineered cells in the future.In this way, Deller et al. synthesized and characterized a new membrane active thrombin construct and demonstrated its application to drive in situ fibrin formation in the plasma membranes of stem cells. The scientists showed that thrombin-based fibrin hydrogel constructs prepared using the new protocol supported high levels of cell growth and viability to eventually produce a self-supporting tissue engineered construct. The stem cells were also able to differentiate along the well-defined adipogenic and osteogenic pathways while demonstrating Young’s moduli similar to the native cells to indicate high levels of integration of the modifications. Deller et al. propose to optimize the protocols for further experiments in vitro prior to in vivo translation, to gain further insight to the enzymatic activity of cell membrane bound bioengineered proteins to develop biocompatible, hemostatic products. Evaluating rhodamine (rh) labelled rh_thrombin, rh_sc_thrombin and [rh_sc_thrombin][ox890] on hMSC plasma membrane affinity. Cells labelled with CellMask (green) and rhodamine labelled thrombin (magenta) visualized with confocal microscopy. Video shows the rh_sc_thrombin [ox890] labelled hMSCs supplemented with fibrin gel conjugating with fibrinogen (green) to highlight fibrin formation emanating from the bioengineered cell surface. Credit: Nature Communications, doi: 10.1038/s41467-019-09763-0 , Science This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.last_img read more