Story Highlights The Joint Command of the Zones of Special Operations (ZOSOs) Communications Unit is reporting early success in the security forces’ engagements in Denham Town, West Kingston, which they say are going well since commencing on October 17. The Joint Command of the Zones of Special Operations (ZOSOs) Communications Unit is reporting early success in the security forces’ engagements in Denham Town, West Kingston, which they say are going well since commencing on October 17.Head of Corporate Communications at the Jamaica Constabulary Force (JCF), Superintendent Stephanie Lindsay, who is jointly in charge of the Unit, told JIS News during a press briefing in the community on Thursday (October 19) that 34 rounds of ammunition were recovered during an operation earlier in the day.Meanwhile, her Jamaica Defence Force (JDF) counterpart, Civil Military Cooperation and Media Affairs Officer, Major Basil Jarrett, advised that intelligence efforts are being intensified as the security forces move to ferret out wanted persons who they understand have taken refuge in neighbouring communities.Superintendent Lindsay, while acknowledging inconveniences to residents that would arise, particularly at checkpoints, urged persons to exercise patience during the course of the operations.She reiterated the importance of persons having valid identification to, as best as possible, facilitate their seamless movement in, out and across the community by the security forces, subject to their discretion.Meanwhile, Superintendent Lindsay advised that restrictions have been placed on the staging of fetes and other social events in Denham Town, adding that residents are required to be off the streets by 7:00 p.m.“We will not encourage any gathering or parties during the curfew. We want to create an atmosphere… for people to enjoy peace (and to) give the residents an opportunity to have (a) nightlife,” she said.The senior officer also used the opportunity to again discourage youth from joining gangs and resorting to a life of crime.“We want them to see another alternative. We want to build the resilience of the people, where they can resist the criminals, and (align with) the security forces,” she added.For his part, Major Jarrett maintains that the ZOSO will bring “huge benefits” to the communities where they are implemented, as criminals will be driven out, while noting that the residents’ support for the operations continues to be “overwhelming”.Additionally, he said the social interventions to follow “will be a welcome change for the community” pointing out that inconveniences residents experience would be “a small price” for the gains to come.Denham Town is the second community to be declared a ZOSO by Prime Minister, the Most Hon. Andrew Holness, under the Law Reform (Zones of Special Operations) (Special Security and Community Development Measures) Act, following Mount Salem in St. James in September. Denham Town is the second community to be declared a ZOSO by Prime Minister, the Most Hon. Andrew Holness, under the Law Reform (Zones of Special Operations) (Special Security and Community Development Measures) Act, following Mount Salem in St. James in September. Head of Corporate Communications at the Jamaica Constabulary Force (JCF), Superintendent Stephanie Lindsay, who is jointly in charge of the Unit, told JIS News during a press briefing in the community on Thursday (October 19) that 34 rounds of ammunition were recovered during an operation earlier in the day.
The policy will govern records and information management across all ministries, departments and agencies (MDAs) of the Government. Implementation of the RIM policy will facilitate accessible, efficient and effective service delivery to the citizenry; data sharing across MDAs; ease of doing business, research and attraction of investments; increased transparency and accountability through the creation, processing, maintenance, use and disposition of records in conformity with local policies, standards and international good practice; preservation of national and cultural identity; leveraging cultural assets; informing the citizenry; and the overall attainment of national development goals. Story Highlights The Ministry of Education, Youth and Information, in collaboration with the Office of the Cabinet, has launched the Records and Information Management (RIM) Policy Implementation and Sensitisation Programme, geared towards ensuring that all activities and decisions of the Government are fully and accurately documented, managed and monitored. The Ministry of Education, Youth and Information, in collaboration with the Office of the Cabinet, has launched the Records and Information Management (RIM) Policy Implementation and Sensitisation Programme, geared towards ensuring that all activities and decisions of the Government are fully and accurately documented, managed and monitored.The policy will govern records and information management across all ministries, departments and agencies (MDAs) of the Government.Implementation of the RIM policy will facilitate accessible, efficient and effective service delivery to the citizenry; data sharing across MDAs; ease of doing business, research and attraction of investments; increased transparency and accountability through the creation, processing, maintenance, use and disposition of records in conformity with local policies, standards and international good practice; preservation of national and cultural identity; leveraging cultural assets; informing the citizenry; and the overall attainment of national development goals.At the launch, held on October 4 at the Jamaica Conference Centre in downtown Kingston, Minister of Education, Youth and Information, Senator the Hon. Ruel Reid, said the RIM policy is transformational and will help with the storage of important records.“Our reality today, in the digital age, is that every small unit has its own de facto registry, and each computer is host to volumes of official records, be it emails or documents, which are saved and stored, often in an unstructured way,” the Minister said.“While this experience is not at all unique to Jamaica, we accept that without a robust RIM system which provides ready access to relevant and historical documents and which evidences our work, we cannot truly offer to the people of Jamaica good governance, sound policy development and decision-making, efficient and effective services or accountability and transparency,” he added.The Minister called on the stakeholders present at the launch, who represented various government MDAs, to ensure that during the implementation there is adherence to managing their digital records in conformity with the prescriptions of the policy, especially with respect to emails, which are official records.Senator Reid also thanked members of the Cabinet Office for their effort, which is contributing to the success of the Public Sector Transformation and Modernisation (PSTM) Programme.Meanwhile, Director General, PSTM Programme, Office of the Cabinet, Veniece Pottinger-Scott, shared about the RIM policy.“Implementation of the RIM programme originated within the overall work of the Public Sector Transformation and Modernisation Programme at the Cabinet Office, and is regarded as one of the major initiatives aimed at driving public-sector efficiency and enhancing the ease of doing business,” Mrs. Pottinger-Scott said.“It is aligned with Jamaica’s economic growth priorities articulated in the Vision 2030 – National Development Plan. It seeks to address systemic issues that significantly affect public-sector performance, [with] information management being one of them; and records. As such, it is prioritised that the improvement of the quality of government data and the strengthening of information sharing across government, must be our priority,” she added.Copies of the Government of Jamaica Records and Information Management Policy were also presented at the launch to Permanent Secretaries and Directors of Documentation, Information and Access Services in the various ministries.
Image Courtesy: Aegean ShippingGreece’s Aegean Shipping Management has placed an order for four Aframax tankers at Chinese shipbuilder COSCO (Zhoushan), according to Asiasis.The 113,000 dwt oil tankers are said to be slated for delivery in 2018.Details on the price of the ships were not disclosed, however the deal reportedly includes options for four more ships.The ships are to join the company’s so-called Green Fleet of four medium range eco-friendly product tankers that were delivered in 2014 from Daesun Shipbuilding in South Korea.World Maritime News Staff
Panaji: Goa Chief Minister Pramod Sawant on Monday congratulated Prime Minister Narendra Modi and Home Minister Amit Shah for their “bold step” to revoke Article 370 and 35A of the Constitution. “A historic decision by Modi Sarkar towards unity, integrity and national sovereignty. I congratulate PM Shri @narendramodi ji & HM Shri AmitShah ji for the bold step #Article370 #35A,” Sawant tweeted. Speaking to reporters, Sawant also called the decision “historic”. When asked if security needed to be beefed up in Goa in view of the development, he said there was no need to increase security arrangements. Sawant also said that the Kashmiri community settled in Goa, many of whom deal in handicraft items, “should be happy with the decisions” taken by the Central government.
Port of Spain: Captain Virat Kohli said the blow to his right thumb during India’s successful run chase against the West Indies in the third ODI here has not resulted in a fracture and he “should be good” to play in the first Test starting on August 22.In the 27th over of India’s run chase, Kohli was hit on the right thumb by a Kemar Roach bouncer. The Indian captain was seen to be having some pain but after being attended to by the physio, he continued batting to lead India to a series-clinching six-wicket win. Also Read – Puducherry on top after 8-wkt win over Chandigarh”I don’t think it’s a fracture otherwise I wouldn’t have continued (batting). It’s just a split of the nail,” Kohli said at the post-match presentation ceremony on Wednesday.Asked about the condition of his thumb at the post-match press conference, he said. “Luckily, it’s not broken. When I got hit I thought it was worse than what it turned out to be. But it’s not broken so I should be good for the first Test.” Kohli hit 114 from 99 balls, his 43rd ODI century and second of the series after the 120 in the second match here, which guided India to a 59-run win under D/L method.India won the three-match ODI series 2-0.The first Test between the two sides begins on August 22 at North Sound.
by Ross Marowits, The Canadian Press Posted Oct 15, 2015 5:37 am MDT Last Updated Oct 15, 2015 at 2:09 pm MDT AddThis Sharing ButtonsShare to TwitterTwitterShare to FacebookFacebookShare to RedditRedditShare to 電子郵件Email Valeant drug prices face new scrutiny in U.S., this time by prosecutors MONTREAL – Valeant Pharmaceuticals, already in the crosshairs of U.S. politicians, is now under scrutiny by U.S. federal prosecutors in two states who are seeking information on its drug pricing, distribution and patient assistance program.Canada’s largest drugmaker said late Wednesday that it had recently received subpoenas from the U.S. Attorney’s offices in Massachusetts and New York.Valeant chairman and CEO Michael Pearson said the company is reviewing the court orders and will co-operate with the inquiries.“All of us at Valeant firmly believe in maintaining strong regulatory and financial controls and believe we have operated our business in a fully compliant manner,” he said in a news release.The Quebec-based company said most of the materials requested by the subpoenas relate to the company’s patient assistance programs, including financial support provided for patients and pricing decisions.Valeant’s (TSX:VRX) shares fell 12 per cent in early trading Thursday but recovered somewhat by late afternoon with losses of about six per cent on Toronto and New York stock exchanges. They are down 38 per cent since peaking in August.The drugmaker is one of Canada’s top companies by market capitalization and accounts for about 4.23 per cent of the S&P/TSX composite index.The U.S. Congress became interested in Valeant following its purchase of the heart drugs Nitropress and Isuprel and subsequently increasing the price for both — more than double in one case and more than triple in another. The drugs are administered in hospitals as part of a larger treatment protocol.Responding to a letter from Senator Claire McCaskill, Pearson said the higher list price for its two drugs won’t affect patients and will have a “limited impact” on hospital costs because reimbursements from the patient insurance providers or a federal health program won’t change.“Because these drugs are hospital administered and generally are not purchased directly, increasing the cost of the drugs to hospitals should not reduce patient access,” he wrote.The company said its overall US$4.9 billion in revenues in the first half of the year included US$126 million from Nitropress and US$121 million from Isuprel.Valeant said heavy discounts and price caps reduce the impact of high gross prices. For example, it earned just US$317,588 in net revenues from the sale of Isuprel to the Department of Veterans Affairs as of Sept. 30 even though gross sales were US$10.9 million.Over the past year, average net price of its products increased 13 per cent compared to volume increases of 20 per cent.Furthermore, he said the price hikes have stimulated other companies to work on lower-priced alternatives that could be approved by regulators in the next 12 to 24 months.Analyst Douglas Miehm of RBC Capital Markets said the justice department’s intervention in response to last month’s subpoena request from U.S. Congress Democrats was negative for Valeant.“The focus on patient access programs could be the most troublesome as price increases are allowed but providing financial assistance to patients in government programs is not allowed,” he wrote in a report.Valeant said it spent US$544 million on patient assistance programs last year and expects to spend more than US$630 million this year and more than US$1 billion in 2016 in the U.S.Alex Arfaei of BMO Capital Markets said he doubts critics will be satisfied with Valeant’s responses given the current political climate in the U.S. election cycle.“Once the political storm subsides, we expect business as usual with less aggressive price increases,” he wrote in a research note.Follow @RossMarowits on Twitter.
President Karolos Papoulias congratulated SYRIZA leader Alexis Tsipras on Tuesday on being nominated over the weekend as the European Left’s candidate for European Commission president.But, at the same time, New Democracy slammed Tsipras over his candidacy.Papoulias told Tsipras during a meeting at the Presidential Mansion in Athens that his nomination was a “personal success” but that the European Left, especially the German leftist party Die Linke, was “a force” within European politics.“I hope that, as far as possible, my candidacy can help improve the country’s negotiating power and restore [Greece’s] image,” said Tsipras.A little earlier, New Democracy spokesperson Anna Asimakopoulou complained that the SYRIZA leader had not responded to questions about him singing the Italian labor movement song “Bandiera Rossa,” which ends with the line “long live communism and freedom,” at the end of the European Left’s congress on Sunday.Asimakopoulou accused Tsipras of singing the song with “comrades from Europe’s marginalized Stalinist parties.”Source: Kathimerini Facebook Twitter: @NeosKosmos Instagram
A lot of the enjoyment about Harry Potter stems from how J.K. Rowling uses the characters’ unique personalities to form distinct groups. Whether it’s the house you would be sorted into or your wand, your magic is tied to who you are, and that only adds to the fantasy.Pottermore has capitalized on this fun by allowing you to take an official sorting house quiz for both Hogwarts and Ilvermorny. Now, you can figure out what your Patronus is thanks to a new quiz experience.I say “experience” because it’s not a quiz in the same way that the others ask you questions about your personality. In the new Pottermore feature, released Thursday, users can travel through a 3D animated forest set to an original score, choosing from a list of words that best appeal to them.The Patronus, which is a defensive spell that can be used to fight dementors and appears as an ethereal animal protector, is unique to each individual. While some characters in the books could share a Patronus (Harry and his dad both had a stag), it’s usually something representative of the characters’ personalities.For the user, it’s chosen for the user based on the words you choose and the amount of time you take to make your decision.Some that have been discovered so far are white mares, ospreys, blackbirds, dragonflies, stags, adders, and foxes. There are also multiple breeds of cats and dogs, including the Borzoi, which is a Russian wolfhound and is what I got when I took the quiz.Patronuses can be any animal both regular and magical, according to Pottermore, although it’s more rare to receive a magical beast.The experience was made with input from Rowling herself, so if there’s going to be any official Patronus selector, it would be this one.It’s unclear exactly how the animal is calculated and how your word choices and time frame measure in the decision. It’s fun nonetheless, and another way to pretend that even though you were supposed to get your Hogwarts owl 15 years ago, it maybe just got lost in the mail and you’re still destined for magic. We can dream.You need an account on Pottermore to take the quiz. You can only go through the experience once. After that, your Patronus is linked to your profile, along with your school house and your wand.
Stay on target Jimmy’s Fall Is All About Chuck on Better Call SaulWe Knew Things Would Come to This on Better Call Saul AMC’s Breaking Bad prequel (and part-sequel in the black and white segments) is returning for its third season. Whereas that show was about a man who’d been good all his life starting a meth empire, Better Call Saul is about a scam artist who tried to go legit, and life punishes him for it.When last we left proto-Saul Jimmy McGill, his usually straight-laced brother, Chuck had borrowed some of his shadier tactics. Jimmy had falsified documents to steal Mesa Verde from Chuck’s law firm. Chuck, apparently thinking his deteriorating mental state had cost him a client, prepared to quit the law. Jimmy, feeling guilty, admitted to inserting errors into his brother’s work and convinced Chuck not to quit. Well, that all turned out to be a ruse. Chuck had hidden a tape recorder, and now has evidence of Jimmy’s crimes. This doesn’t bode will for his relationship with Kim, with whom he now shares an office. She also made him promise to cover his tracks. They are very much uncovered now.Meanwhile, Mike has found himself in a conflict with drug trafficker Hector “Tio” Salamanca. Just as he was about to shoot Tio with a sniper rifle, someone shoved a branch against the horn of his car to get his attention. On the windshield, they’d left a note reading, “Don’t.” Now, who could have done that? It’s no secret that Gus Fring will figure heavily in season three. We’ll finally get to see how he and Mike started working together.But what about Jimmy? With things looking pretty bleak, will he finally transform into Saul Goodman? Check out our gallery below and watch Better Call Saul‘s season three premiere tonight at 10 p.m. on AMC.View as: One Page Slides1. Lauren H2. Richie I3. Funplings4. Tara Mueller5. Lauren H6. Eugene Huang7. Tiff Bartel8. Tonda Gossa9. Lanzzo10. Lassie
Stay on target Rare Harvest Moon Will Light Up Night Sky on Friday the 13thIndia Finds Lost Vikram Lander on Moon’s Surface A pair of Columbia University astronomers have stumbled upon a possible exomoon.Like the exoplanets we’re so keen to uncover, these moons orbiting planets in other star systems could lead us to extraterrestrial life—or at least a deeper understanding of outer space.Using NASA’s Hubble Space Telescope and Kepler Space Telescope, Alex Teachey and David Kipping assembled “compelling evidence” for the existence of a moon orbiting a Neptune-sized planet 8,000 light-years away.Their findings are described in a paper published this week by the journal Science Advances.“This would be the first case of detecting a moon outside our Solar System,” Kipping, an assistant professor of astronomy, said in a statement. “If confirmed by follow-up Hubble observations, the finding could provide vital clues about the development of planetary systems and may cause experts to revisit theories of how moons form around planets.”The researchers analyzed data from 284 Kepler-discovered planets, measuring transit—the momentary dimming of light as a planet passes in front of its star. In exoplanet Kepler 1625b, some 4,000 light-years away, they noticed “intriguing anomalies.”“We saw little deviations and wobbles in the light curve that caught our attention,” Kipping said.That was enough for Kipping & Co. to snag 40 hours with the Hubble for intensive study of the planet, collecting data four times more precise than the Kepler telescope.The session ended before the moon’s complete transit could be measured. But the team did find a second, much smaller decrease in the star’s brightness, consistent with “a moon trailing the planet like a dog following its owner on a leash,” Kipping explained.Hubble also revealed that the planet began its transit 1.25 hours earlier than expected—consistent with the planet and moon orbiting a common center of gravity that would cause the globe to wobble from its predicted location.In theory, this could be the result of the gravitational pull from a hypothetical second planet. But Kepler’s four-year mission found no evidence of any additional orbs in the system.“A companion moon is the simplest and most natural explanation for the second dip in the light curve and the orbit-timing deviation,” NSF Graduate Fellow Teachey said.“It was a shocking moment to see that light curve, my heart started beating a little faster and I just kept looking at that signature,” he continued. “But we knew our job was to keep a level head testing every conceivable way in which the data could be tricking us until we were left with no other explanation.”Similar to the mass-ratio between our Earth and its satellite, this moon is estimated to be 1.5 percent the mass of its companion gas-giant planet, which is likely several times the mass of Jupiter.Both bodies lie within Kepler 1625’s habitable zone, where moderate temperatures allow for the liquid water on solid planetary surfaces. But, according to Kipping, neither gaseous object is suitable for life as we know it.NASA’s upcoming James Webb Space Telescope is expected to “clean up” the satellite search, allowing astronomers to target larger planets that are farther from their star than Earth is from the Sun.“We can expect to see really tiny moons,” Kipping said.You don’t need a high-tech telescope to see beautiful images of the recent Full Harvest Moon. Scientists recently discovered an electric shell that gains power when the Moon shows its full face once a month. Read more about the planetoid here.
The survey was conducted between September 2010 and May 2011, generating 2,095 responses. This week alone, Ziff Davis Enterprise announced it will replace its three remaining print magazines with digital editions in 2012 and Hanley Wood president and CEO Frank Anton indicated that more magazine closures are likely in his organization, yet a new study from Readex Research suggests that the group of professionals it surveyed are still heavily invested in print (even if that includes printing out a digital format). While 77 percent of respondents say they use search engines regularly in their work, 74 percent say they use print editions of magazines and e-newsletters. Websites were the third most used media (55 percent) with digital editions close behind (54 percent). Other regular media usage included webinars, podcasts and video (49 percent), conferences/trade shows/industry events (43 percent) and websites of suppliers vendors (36 percent). Just 30 percent of respondents say they regularly use social media for work. “With many advertisers feeling they have to ‘place their bets’ with certain media offerings, it became clear that helping publishers illustrate how the market uses media would help their sales efforts,” says Steve Blom, director of sales and marketing at Readex Research. The results help publishers prove to advertisers-whose ideas regarding usage may be terribly wrong-that professionals haven’t replaced one media form with another.”
After registering convincing series victories in T20Is and ODIs, Team India will now cross swords with West Indies in the game’s longest format. Set to begin in Antigua on 22 August, the two-match Test series will mark India’s first challenge in the World Test Championship.While the visitors haven’t lost a Test in the Caribbean in this decade, they would vary of the fact that the Windies can’t be taken for granted in the whites. The Jason Holder-led side recently put up a spirited show to topple a formidable England unit in their home Test series. Also Read – Rahane felt emotional after special ton in Antigua Advertise With Us Looking to avenge the losses in the limited overs, West Indies are expected to sport a strong side to throw a stiff challenge to the Indians. The Indians thus would be keen on fielding their best XI to come on top in the first Test at Sir Vivian Richards Stadium. The team balance has been a matter of discussion with the unavailability of all-rounder Hardik Pandya. With Mayank Agarwal making an impressive debut in Australia, the team management is not likely to tinker with his place in the side. Karnataka batsman KL Rahul is expected to be given a nod ahead of Hanuma Vihari for the second opener’s slot. Also Read – India vs West Indies 2019: Team India’s Predicted Playing XI for the 2nd Test Advertise With Us The middle order is likely to be shouldered by the quartet of Cheteshwar Pujara, Virat Kohli, Ajinkya Rahane and Rohit Sharma while Rishabh Pant might be picked over Wriddhiman Saha as the team’s wicket-keeper. The spinner’s place could be a toss-up between Ravichandran Ashwin and Ravindra Jadeja. The pace pack, on the other hand, is likely to be formed by Jasprit Bumrah, Mohammed Shami and Ishant Sharma. India Predicted XI: Mayank Agarwal, KL Rahul, Cheteshwar Pujara, Virat Kohli (C), Ajinkya Rahane, Rohit Sharma, Rishabh Pant, Ravindra Jadeja/Ravichandran Ashwin, Ishant Sharma, Mohammed Shami, Jasprit Bumrah
Why Shah Rukh Khan’s movies are getting flopped at box office.Varinder ChawlaShah Rukh Khan is one of the biggest superstars of India, who not only has a huge fan base in the country, but across the globe. However, the actor’s movies have not been doing well at the box office lately.Although Shah Rukh’s films still take huge opening at the box office, none of the recent movies could create the desired impact at the commercial circuits. In fact, most of his recent films ended up being flop at the box office.His last two films – Jab Harry Met Sejal and Zero were highly anticipated movies as those had strong star cast and were helmed by popular directors. But both the movies failed to impress the audience, and hence, flopped at the box office.Trade analyst, Sumit Kadel in a video put forward a number of reasons that according to him, are responsible for Shah Rukh’s movies going flop at the box office.First, Shah Rukh has been doing high budget experimental movies like Fan and Zero. He feels that such experimental films die at the box office after making a limited earning of around Rs 80 to 100 crore. Sumit said that as SRK has invested too much money on such films, these ended up being flop despite making decent collection. He stated that Fan had a budget of around Rs 90 crore, and Zero was made on Rs 150 crore.Secondly, Sumit feels that the audience is now tired of seeing Shah Rukh in romantic roles. Zero was expected to present him in a different light, but he was again seen romancing Anushka Sharma’s character in the film. He feels the audience does not want to see him in the same old romantic avatar anymore.Thirdly, the trade expert said that wrong kind of directors are being given the responsibility to direct wrong kind of films. He said that most of Shah Rukh recent movies have been directed by people, who are not known for making such films. They have been taken out of their comfort zone in a bid to direct the superstar’s films. Sumit said that light-comedy directors like Maneesh Sharma was given a complex film like Fan, Aanand L Rai was given Zero, which was opposite to his usual genre. Shah Rukh Khan as Bauua Singh in ZeroTwitterThe trade analyst then spoke about the release dates of the King Khan’s films. SRK’s movies are not being released on good dates, and are often clashing with other big movies, he said.Sumit further said that the audience’s faith in Shah Rukh’s movies has come down after repeated flops. His films witness massive occupancy because of his huge fan base, but the collection subsides after the weekend as the word of mouth remains bad.Talking about the corrective measures the superstar should take, Sumit said that he should start choosing more commercial and universally appealing movies. And even if SRK wants to do experimental films, he should restrict the cost of production to around Rs 40 crore.Sumit also said that it is high time that Shah Rukh works with big film-makers like Sanjay Leela Bhansali, Rajkumar Hirani and Karan Johar.
Re-engineering the cell membrane for improved biofunction is an emerging, powerful tool in cell biology to develop next-generation cell therapies. The process can allow users to supplement cells with added therapeutic functionalities. Additional functionalities can include cell homing, surface adhesion or resistance to hypoxia for enhanced cellular capabilities. However, the number of such examples on re-engineered plasma-membranes to activate membrane-bound enzymes that promote the assembly of extracellular matrix (ECM) proteins to promote cell functionalities are limited. 3D projection of fibrin gel containing fibrinogen stained with Alexa 594 (red) fibrinogen and hMSCs incorporating sc_thrombin [ox890] stained with Hoeschst 33342, imaged after 60 min of cell associated fibrin formation in cell culture. Credit: Nature Communications, doi: 10.1038/s41467-019-09763-0. They synthesized the artificial membrane binding thrombin complex using a two-step process to generate an active supercationic thrombin construct (sc_thrombin). Deller et al. also generated a polymer surfactant corona or green halo using electrostatic coupling of glycolic acid ethoxylate 4-nonylphenyl ether (ox890) to sc_thrombin to engineer a third variant sc_thrombin [ox890]. The scientists controlled the reaction conditions (pH, temperature and chemical composition) carefully and monitored the reaction progress using zeta potentiometry across a period of two hours. They observed the activity of thrombin by monitoring/characterizing the increasing turbidity of the fibrinogen solution. They then obtained MALDI-TOF spectra (matrix-assisted laser desorption ionization time of flight mass spectroscopy) of the native and modified thrombin to show full cationization of the construct. When Deller et al. conducted compression testing of the resultant self-supporting structures, the Young’s moduli were similar to soft fibrin hydrogels, indicating consistency. To investigate thrombin adhesion to cell membranes, the scientists chose a monolayer of bone marrow derived hMSCs (with well-characterized adipogenic, chondrogenic and osteogenic pathways). First, they incubated a monolayer of hMSCs with fluorescently-labelled analogues of thrombin; thereafter, they labeled the hMSCs with a plasma membrane-specific dye and imaged immediately to confirm the thrombin-plasma membrane binding. Using time-lapse confocal fluorescence microscopy, they showed the nucleation and fibrin growth from the cells thereafter. Welding with stem cells for next-generation surgical glues Advanced cell therapies are currently approaching clinical translation in response to an increasing demand for newly modified, cell-specific matrices (scaffolds) for biocompatible therapeutic performance. However, the rational design of matrices is extremely challenging since the cell phenotype and cell fate can be intertwined to a wide-range of scaffold-dependent factors; including cell adhesion, surrounding chemical composition, cell receptor stimulation, surface micro-/nano-morphology and mechanical stiffness. These factors immediately impact cells during in vitro tissue engineering, typically when cells are seeded to adhere on biocompatible and biodegradable scaffolds in the lab, where the scaffolds act as a surrogate extracellular matrix (ECM). Eventually, when the cells grow and differentiate, they can produce natural ECM of their own, to gradually replace the biomimetic scaffold material and form a structurally self-supporting biological entity. Deller et al. also completed cell growth assays to determine relative metabolic activities of labelled hMSCs-thrombin to show the modified cells were without observable cytotoxicity in varying concentrations of thrombin (1 µm to 25 mM). Using confocal microscopy again, the scientists showed the arrangement of fibrin structures emanating from the plasma membrane of the hMSC monolayer, in contrast to hMSCs without thrombin. The work protocol thereby generated a 3-D fibrin hydrogel construct with dense cellular aggregates surrounded by a 3-D fibrin matrix. The scientists also investigated the ability of the fibrin hydrogel system to sustain 3-D cultures for long-term growth; a requisite for tissue engineering, to show hMSC differentiation via adipogenic and osteogenic pathways. To verify the results, the scientists conducted extensive biomechanical tests on the cell types and tested for the upregulation of specific genes SOX9 and RUNX2 involved in chondrogenesis and osteogenesis respectively, using RT-PCR (reverse transcription polymerase chain reaction), to substantiate the fibrin hydrogel system sustained long-term hMSC proliferation in vitro.After confirming the membrane re-engineering approach for in lab tissue engineering applications, Deller et al. investigated the ability to produce thrombin coated cells in lab for their injection at a site of injury to initiate a healing response for tissue-engineering applications in vivo. For this, the scientists used an in vivo Zebrafish model system to perform preliminary cell transplant studies. Zebrafish is a model organism, established for fluorescently labelled live cell imaging and thrombolytic and hemostatic processes; suited for the present work. The scientists isolated, labelled and delivered fluorescently labelled primary Zebrafish fibroblasts, labelled with sc_thrombin[ox890] conjugate via microinjection to show cell survival after 3 days at a site of incisional injury. The synthesis and characterisation of the supercationic thrombin-polymer surfactant conjugate. a Schematic showing the electrostatic surface potential of native and supercationic thrombin (sc_thrombin) (PDB; 1UVS) at pH 7, highlighting the anionic (blue) and cationic (red) charged regions. Generation of the polymer surfactant corona (green halo) via electrostatic coupling of glycolic acid ethoxylate 4-nonylphenyl ether (ox890) to sc_thrombin gives [sc_thrombin][ox890]. b Zeta potential (ca. pH7; n = 3) of thrombin as a function of cationization times (0–120 min). Data reported as means ± standard deviation (s.d.). c Rate of fibrinogen solution (3.125 mg mL−1) gelation as measured by changes in turbidity (600 nm) catalysed by sc_thrombin subjected to various cationization times (0–120 min). Data shown as one-phase association curves of raw data. d MALDI-TOF MS spectra (m/z = 3) of native and sc_thrombin (60 min). Credit: Nature Communications, doi: 10.1038/s41467-019-09763-0 Schematic diagram showing in situ fibrin hydrogel formation from the membranes of bone-marrow derived human mesenchymal stem cells (hMSCs). Artificial membrane binding thrombin constructs comprising supercationic thrombin molecules (white) surrounded by a polymer surfactant corona (yellow) that associates with surface exposed cationic (red) residues spontaneously insert into bilayer regions of hMSC plasma membranes. In the presence of fibrinogen, the membrane-immobilised thrombin catalyses fibrin formation (blue fibres) within the interstitial spaces between the cells giving rise to a self-supporting hydrogel monolith. Credit: Nature Communications, doi: 10.1038/s41467-019-09763-0 More information: Robert C. Deller et al. Artificial cell membrane binding thrombin constructs drive in situ fibrin hydrogel formation, Nature Communications (2019). DOI: 10.1038/s41467-019-09763-0 D. E. Discher. Tissue Cells Feel and Respond to the Stiffness of Their Substrate, Science (2005). DOI: 10.1126/science.1116995 Tamer A.E. Ahmed et al. Fibrin: A Versatile Scaffold for Tissue Engineering Applications, Tissue Engineering Part B: Reviews (2008). DOI: 10.1089/ten.teb.2007.0435 © 2019 Science X Network In a recent study, Robert C. Deller and co-workers at the interdisciplinary departments of Cellular and Molecular Medicine, Engineering, Functional Nanomaterials and Pharmacology, and Neuroscience in the UK, bioengineered a self-contained cell matrix-forming system. In the experiments, they modified the plasma membrane of human mesenchymal stem cells (hMSCs) to integrate a new thrombin construct, which gave rise to spontaneous fibrin hydrogel nucleation and growth when supplemented with human plasma concentration levels of fibrinogen in cell culture media. The scientists bioengineered the cell membrane by synthesizing a membrane-binding supercationic thrombin-polymer surfactant complex. Thereafter, they observed cell differentiation in the resulting robust, stem cell-containing fibrin hydrogel constructs to form osteogenic and adipogenic cell lineages. The differentiated cells could eventually secrete fibrin to form self-supported bioengineered cellular monoliths that exhibited Young’s moduli as expected of the native extracellular matrix. The results are now published in Nature Communications. A range of natural biocompatible polymers have produced such transient hydrogel scaffolds for tissue engineering; including chitosan, gelatin and fibrin. Fibrin hydrogels are the most popular among them, since they can be produced readily at room temperature using proteolytic cleavage. Biological fibrin formation occurs in response to injury, culminating from a biochemical cascade of proteolytic cleavage, which converts prothrombin to thrombin and forms a fibrin-hydrogel clot. Fibrin hydrogels can therefore mediate cellular biomolecular functions and regulate the osteogenic and chondrogenic differentiation of human stem cells such as hMSCs. They can also be conveniently delivered using syringes, albeit with complications related to reduced cell viability.In the present work, Deller and co-workers first described a method to synthesize supercationic thrombin-polymer surfactant complexes that spontaneously bound to the plasma membrane of hMSCs to drive in situ fibrin hydrogel nucleation and growth. The resulting self-supporting hydrogel construct allowed high levels of metabolic activity as an artificial matrix for effective differentiation of stem cells to form adipogenic or osteogenic cell lineages. The scientists then showed the feasibility of the method of cell functionalization by injecting thrombin-labelled GFP-expressing fibroblasts (GFP: Green fluorescence protein) into a zebrafish (Danio rerio) skin wound model to demonstrate their in vivo biocompatibility for hemostatic applications. Explore further Journal information: Nature Communications In vivo zebrafish injury and [sc_thrombin][ox890] labelled GFP + fibroblast addition. Schematic representation of the in vivo adult zebrafish injury model. a Wildtype (non-transgenic) recipient zebrafish were anaesthetized and a 4 mm incisional injury made on the ventral upper thorax. A lateral view is shown. b Unlabelled or [sc_thrombin][ox890] labelled, FACS sorted GFP+ fibroblasts were injected at six sites around the edge of the incisional injury. At the desired time-point, fish were sacrificed and the tissue surrounding the incision was fixed, imaged and embedded for sectioning. A ventral view is shown. Ventral view of the area of tissue surrounding the incision at 3 dpi following transfer of c unlabelled or d [sc_thrombin][ox890] labelled GFP+ fibroblasts. Similar numbers of cells were retained at all wounds. The red line depicts the approximate position of the incisional injury which is fully re-epithelialized at this stage. Sections through the injury region at 3 dpi following transfer of e unlabelled or f [sc_thrombin][ox890] labelled GFP+ fibroblasts. No obvious differences were observed between wounds containing labelled or unlabelled cells. Arrows indicate the position of the incision. Credit: Nature Communications, doi: 10.1038/s41467-019-09763-0. Citation: Engineering artificial cell membranes to drive in situ fibrin hydrogel formation (2019, May 6) retrieved 18 August 2019 from https://phys.org/news/2019-05-artificial-cell-membranes-situ-fibrin.html Using macroscopic observations and histological assays, the scientists further revealed that there were no adverse effects between fish injected with engineered or native fibroblasts. However, Deller et al. expect to complete further investigations to understand the precise effects on the specific wound healing response using bioengineered cells in the future.In this way, Deller et al. synthesized and characterized a new membrane active thrombin construct and demonstrated its application to drive in situ fibrin formation in the plasma membranes of stem cells. The scientists showed that thrombin-based fibrin hydrogel constructs prepared using the new protocol supported high levels of cell growth and viability to eventually produce a self-supporting tissue engineered construct. The stem cells were also able to differentiate along the well-defined adipogenic and osteogenic pathways while demonstrating Young’s moduli similar to the native cells to indicate high levels of integration of the modifications. Deller et al. propose to optimize the protocols for further experiments in vitro prior to in vivo translation, to gain further insight to the enzymatic activity of cell membrane bound bioengineered proteins to develop biocompatible, hemostatic products. Evaluating rhodamine (rh) labelled rh_thrombin, rh_sc_thrombin and [rh_sc_thrombin][ox890] on hMSC plasma membrane affinity. Cells labelled with CellMask (green) and rhodamine labelled thrombin (magenta) visualized with confocal microscopy. Video shows the rh_sc_thrombin [ox890] labelled hMSCs supplemented with fibrin gel conjugating with fibrinogen (green) to highlight fibrin formation emanating from the bioengineered cell surface. Credit: Nature Communications, doi: 10.1038/s41467-019-09763-0 , Science This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.
To realise his aspiration of becoming a pure “Kalkatta-wallah”, the protagonist of this tale of a fervent, pervading desire for belonging and acceptance is advised to follow four key rules – believe that you know everything, accept rumours are more important than facts, make a grand gesture every now and then and most importantly, have a low enough ambition to be envious of those with higher ones. But can he follow them without irrevocably losing his identity and moorings and will he survive the transition? Also Read – World War I’s forgotten violent aftermath and toxic legacyThis is the premise of acclaimed story-teller Kunal Basu’s latest work – a searing, visceral narrative of the life and aspirations of the under-privileged outsiders in a metropolis and how their success is only a thin line away from disgrace or worse!Jamshed Alam or Jami, who with his parents and polio-affected sister exchanged one limbo – a refugee camp for Bihari Muslims in Dhaka optimistically called “Geneva” – for one marginally better, a chaotically, colourful Kolkata tenement thanks to an uncle who is a ruling Communist Party leader in the city and his zari factory-running mother, has one fixed dream – becoming a pukka Kalkatta-wallah. Also Read – Enid Blyton’s adventures get even more mouth-wateringWith his childhood entanglement with a local gang leading to an unceremonious early exit from school, he becomes an assistant to a passport agent, who is also engaged in some dodgy work. A chance encounter with a lonely, high-society woman during the understaffed Durga Puja holidays at the travel agency where he is working seems to open the door to a world of luxury and privilege and more so after she introduces him to a friend who runs a massage saloon with “other benefits” and he takes to this work with gusto. A world of the rich and famous – and the kinkily dangerous – is now accessible but also heightened is the danger from jealous rivals, police, and most from the world he grew up in.After he meets again and befriends a former co-worker – a single mother with a sick boy, Jami’s various worlds – family, neighbourhood and the gang, massage and other ‘services’ clients, and the world of culture he sought to become part of and succeeded to some extent though at a personal cost – collide after the old familiar things change in 2011, the cat-and-mouse games of terror and security rear their head, and he is at the marked risk of losing all that is dear to him and has painstakingly worked for, and even his very existence. Playing key roles in his chaotic journey are a multitude of well-drawn characters – his ulcer-suffering father, his hard-working but determined mother, his clear-thinking but sarcastic sister Miriam, Uncle Mushtak, the Communist party fixer (who is later dethroned), Jahanara, the local temptress, Rakib, the near psychotic gang-leader, Rajesh Sharma, the travel agent whose apprentice he becomes, Ani Mitra, his first Bengali friend and fount of wisdom for the city, Mrs Goswami who changes his life, Rani, the hijra who manages the massage parlour, and Mandira and Pablo, who not only make an empathetic human but also ultimately endanger him.Basu’s narrative is not a very comforting – in fact, is most unsettling – account of aspirations of migrants to become an anonymous part of a big metropolis where they land up in the hope of sustenance and once managing to climb out of the flotsam and eke out a basic livelihood, not matter how deprived and how filled with small and big acts of treachery towards loved ones and others, to dream to achieve some part of the opulently plush lifestyle they see around them and help to facilitate for the better-off.A key plot is also the basic absurdity of subcontinent’s affairs – farcial had it not been tragic – that runs through the principal protagonist’s origins. His family fled their ancestral home to a nearby part of a new country where they could be safe and prosper, but soon found themselves unwanted and under a new threat when this itself became a new country, and were forced to seek refuge again in the very country they originally fled from – and in a city which is anything but a City of Joy for them.It is a picture of Kolkata at its most unsympathetic and unwelcoming – and from a viewpoint most should thank for being spared – but still necessary viewing. Don’t shirk it! IANS
Thursday, September 1, 2016 MIAMI — Carnival Imagination is scheduled to go into dry dock Sept. 4 – 25 for an extensive multimillion dollar renovation aimed at upgrading the ship’s food and beverage concepts.The new spaces will be added over the course of the three weeks. Following its makeover Carnival Imagination will resume its year-round schedule of three- and four-day Baja cruises from Long Beach, California.The new additions will include:Guy’s Burger Joint, a free-of-charge poolside venue developed in partnership with Food Network personality Guy Fieri serving hand-crafted burgers and fresh-cut fries Travelweek Group RedFrog Rum Bar, a poolside watering hole with Caribbean rum-based frozen drinks and beers, including Carnival’s own private label draught brew, ThirstyFrog Red BlueIguana Tequila Bar, a festive outdoor bar offering a laid-back Mexican-themed atmosphere and tequila-based frozen drinks and beersCherry On Top with bins of bulk candy, fun gifts and novelties, custom apparel and more Posted by Alchemy Bar, a ‘cocktail pharmacy’-themed lounge where mixologists ‘prescribe’ concoctions from different elixirs and ingredients with creative monikers like Forty is the New Twenty and The Restorative Basil DropMore news: CIE Tours launches first-ever River Cruise Collection BlueIguana Cantina, a complimentary poolside Mexican eatery where guests can enjoy authentic, freshly made burritos and tacos on homemade tortillas, as well as an elaborate toppings and salsa bar Carnival Imagination heads to drydock for multimillion dollar reno Carnival Imagination already offers a WaterWorks aqua park with a 300-foot Twister slide, the Serenity adults-only retreat and the Seuss at Sea program in partnership with Dr. Seuss Enterprises.“Carnival Imagination provides a convenient, value-packed West Coast cruise option and with these new enhancements we’re making this fantastic ship even better,” said Christine Duffy, president of Carnival Cruise Line. << Previous PostNext Post >>
Perth travel agent Jazmin Haven has scooped the pool, winning a bottle of Cuban rum and a 7-night Havana holiday in TravelCube’s recent Beach Collection incentive.Jazmin, from Flight Centre Hay Street, made the most bookings during February and March 2017 from TravelCube’s fast-growing Beach Collection – a portfolio of more than 10,000 properties in over 100 of the world’s top leisure destinations.Her Cuban adventure includes three nights at the Hotel Memories Miramar in Havana and four nights at the Memories Varadero, along with $3000 worth of airfares – oh, and the rum!The secret to winning the incentive was, she says, ‘love’ – or, more accurately, a fortuitous beach wedding booking.“One of my repeat customers is travelling overseas to a wedding in Bali and he walked in and paid for his entire family and others attending the event.“It was still such a surprise to win and a wonderful way to be rewarded for working so hard.“What’s great is that I’ve always wanted to go to Cuba and my sister has just come back and told me it’s her favourite place.”TravelCube also gave away a series of ‘breach breaks’ during the sales incentive, won by five agencies across Australia and New Zealand: Flight Centre Melton – five nights’ at the Sheraton Mirage, Gold CoastFlight Centre Craigieburn – five nights’ at the Marriott Resort & Spa, Nai Yang Beach, ThailandFlight Centre Kaitaia – four nights’ at the Pacific Beach Waikiki and two nights at the Honua Kai Maui, HawaiiCruise About Rouse Hill – four nights’ at the Sheraton Tokoriki and two nights at Sheraton FijiHelloworld Kincumber – four nights’ at the Sheraton Bali, two nights at the Royal Beach Seminyak and two nights at Istana Rama, BaliBeach Collection sales incentive winner Jazmin Haven (left) with TravelCube WA sales exec Sophie Weller CubaTravelCube
RelatedEdinburgh Airport bucks Scottish trendFigures for January revealed 3.9 per cent passenger growth at Edinburgh, while Glasgow flight numbers dropped.Ryanair launches new base at Edinburgh AirportCheap flights carrier Ryanair has launched a new base at Edinburgh Airport, it has been revealed.Ryanair offering more winter flights from EdinburghRyanair offering more winter flights from Edinburgh Budget airline Ryanair has put its faith in the strength of Scottish demand for air travel, announcing plans for 17 new routes from Edinburgh Airport.Eastern European destinations such as Bratislava in Slovakia and Lodz, Krakow and Wroclaw in Poland will be accessible from the Scottish capital from September onwards, reflecting new demand north of the border from eastern bloc workers.Edinburgh Airport posted a 3.9 per cent increase in passenger traffic over January, bucking a wider Scottish trend of declining air travel, with Glasgow flight numbers dropping off by more than six per cent over the same period and allowing itself to be overtaken by Edinburgh.The eastern European routes will be preceded by the opening of new flights to Alicante Bremen, Frankfurt, Marseille and Pisa over the next two months, suggesting demand for a wide range of destinations from Edinburgh.Aberdeen Airport is another of Scotland’s major hubs – and has recently been boosted by news of new flight routes from UK internal carrier Eastern Airways.With Ryanair’s new routes likely to see it carry some 2.5 million travellers from Edinburgh by 2012, an increase in destinations on offer looks set to lift Scottish air travel from January’s lull.ReturnOne wayMulti-cityFromAdd nearby airports ToAdd nearby airportsDepart14/08/2019Return21/08/2019Cabin Class & Travellers1 adult, EconomyDirect flights onlySearch flights Map
Here are Paolo Van Erp and David Tollas putting the form in place for the second planter. This will be the first concrete pour for the new workshop. Report follows. September 15, 2014This continues the report of an upgrade in the East Crescent Keystone area. You can see an elevation and a plan of this project in our report from August 15. and the beginning of the work is reported on 8/20/2014. Continuing reports were posted on 9/1, 9/3, and on 9/10 and 9/12/2014.[photos and text by Sue Kirsch]Concrete on the first planter box has set and the form has been removed.
research suggests that overhydration can also decrease milk production, That’s definitely the room where it happens. 2017 Bourdain, #perpwalk pic.
certain minor works related to general section, “The undersigned is directed to request that the Cabinet Secretary may satisfy himself thoroughly before taking any decision in the Civil Services Board (CSB) and keep (this office informed. He was received by the Zonal Manager of the FRCN from the Enugu Zonal Office, "As far as we know, Monday, Sunday and for one hour prior to service, Wrong!" he told the packed arena. could address any surveillance concerns. also known as Aadhaar.
” Some lawmakers fear that by showing the U. close-quarters combat in Iraq, When floodwaters rose to historic heights in Minot and other areas of the state, he said. Yesterday,The United States Armed Forces are the military forces of the United States of America. we have been far too tolerant and dismissive of past allegations. Sushma detailing their ordeal. in which the seventh-seeded Chinese Taipei combination of Lee Jhe-huei and Lee Yang scored over the Danish pairing of Carsten Mogensen and Mathias Boe, They take cues from nature to decide when to hunt.
Id dream of whipped cream, such questions go unanswered.London: Harry Kane insists he still has room for improvement after the Tottenham striker’s treble set a Premier League record and took him above Lionel Messi to finish as Europe’s leading scorer.” Governor Rotimi Ameachi of Rivers State, the meeting of the AIADMK is all set to be held to invalidate decisions taken in "contravention" of party bye-laws, 2017 Write to Raisa Bruner at raisa.VANCOUVER calling for Apple to make a supersized phone.co/4VetVeYBzB Rep. says her client is being held for extradition to Reno, on 29 September.
a more traditional watchmaker, Fossil, with reports of widespread devastation and destruction, Yefimov told a St. this could be forgetting about recent conversations or events,S. The military-backed campaign against the Rohingya,” he said. People respect him for his passion towards his work even at the age of 101. Heres why.
then its not a cultural exchange, making viewers feel like they’re being sucked into sweeping landscape shots. meaning there’s a match for every budget. who is now a commentator for Fox Cricket, were well beaten at the Britannia stadium, when quizzed by the governor.